内生微生物组对滇龙胆道地性的影响及作用机制的研究

滇龙胆是国家三级重点保护野生药材物种，在云药产业开发中具有重要地位。研究滇龙胆道地性及其形成机制是从源头解决优质高产问题的关键之一。国内外对滇龙胆道地性研究集中于化学、遗传、药理及生态环境等，对滇龙胆内生菌在道地性形成中的作用研究较少。我们前期研究表明内生菌可能不是单独，而是以菌群形式对滇龙胆道地性发挥作用。结合植物微生物组学研究成果，本项目提出内生微生物组对滇龙胆道地性影响及其作用机制的假说，拟从内生菌群整体的微生物组的角度，深入解析内生菌与滇龙胆道地性的关系。通过分析内生微生物组和道地性指标的对应关系，比较内生微生物组组成与结构，结合滇龙胆关键酶活性、基因表达、生理生化等，对全微生物组进行关联和网络分析，阐明内生微生物组对滇龙胆道地性的影响及作用机制，从内生微生物组的角度诠释其未知的生态学功能。该研究为创新中药道地性研究思路、方法提供科学证据，对药材生产实践的生物调控具有重要指导作用。

Gentiana rigescens Franch. plays an important role in the medicine industry of Yunnan, which is one of national three-level important species of wild medicinal materials. It is one of the keys to solve the problem of high quality and high yield from the source to study the geomorphism and formation mechanism of Gentiana rige . The geomorphism research of Gentiana rigescens focused on chemistry, heredity, pharmacology and ecological environment. It is few to research the role of endophytes in the formation of Gentiana rigescens.. Our previous studies have shown that endophytes may play a role in the geomorphism of Gentiana rigescens by groups, not single. Based on the research results of plant microbiome, the hypothesis of the effect of endophytic microbiome on the geomorphism and its mechanism was put forward in this project.. The relationship between endophytic microbiome and geomorphism of Gentiana rigescens will be analyzed deeply based on the view of the whole microbial groups.. The corresponding relationship between endophytic microbiome and geosyncratic index will be analyzed, and the composition and structure of endophytic microbial groups were compared. Combining with the key enzyme activity, gene expression, physiology and biochemistry of Gentiana yunnanensis, the correlation and network analysis of the whole endophytic microbiome were carried out. . The effect of endophytic microbial group on the geomorphism of Gentiana rigescens and its mechanism were elucidated. The unknown ecological function of endophytic microbial group was explained by the endophytic microbial group. . This study will provides scientific evidence for the innovation of ideas and methods for traditional Chinese medicine research, and plays an important role in guiding the biological regulation and production practice of traditional Chinese medicine.