吸血蠓虫可引起蠓咬性皮炎及瘙痒症，并能传播人、畜疾病，许多种类与人类关系密切。运用DNA序列进行物种鉴定和分类越来越受到关注，DNA分类已成为当前生物系统学领域的研究热点和前沿领域，是系统学研究的发展方向之一。本项目拟选择吸血蠓虫为研究对象，选用COI、Cytb、18srRNA和ITS四个分子标记，对三大吸血蠓属(库蠓属、蠛蠓属和细蠓属)代表类群进行DNA分类学研究，通过四个分子标记的序列比对，鉴定形态学上难以分类的物种，揭示隐存分类单元，澄清代表类群中物种系统地位存疑的问题；初步建立属间、属内种间和种内DNA差异标准；构建三大吸血蠓属代表类群的DNA分类数据库，以及重要媒介物种的DNA条形码库，为DNA分类与物种的快速鉴定奠定基础；基于上述类群的代表物种，利用四个基因的联合分析，探讨上述三属及其所代表的亚科间的系统发育关系。本研究将为媒介昆虫的系统进化、生物多样性和物种鉴定提供基础数据。

The insects of blood-sucking midges can cause dermatitis and itch disease, and can spread diseases of people and livestock. So many species of Ceratopogonidae have close relationship with humans. People are concerned about identification and classification based on DNA sequences, which is research focus and front in the field of biosystematics. The research incluses DNA taxonomy based on molecular markers (COI, Cytb, 18srRNA and ITS) from blood-sucking midges, through sequence alignment of 4 molecular markers we can identify species, reveal cryptic taxa, clarify taxonomic status. The standard of DNA difference will be established from the genera, species and intraspecies. DNA classification database and DNA barcoding will be the foundation for DNA taxonomy and species identification. Based on the conjoint analysis of 4 gene, phylogenetic analysis will be study about representative species from the genus Culicoides, Leptoconops and Lasiohelea. The result will provide basic material for system evolution, biodiversity and species identification of media insect.