玉米细胞壁相关激酶ZmWAK介导的丝黑穗病数量抗性分子机理

玉米丝黑穗病是由丝轴黑粉菌（Sporisorium reilianum）引起的一种真菌性土传病害，在世界各地均有发生，对玉米生产构成严重威胁。遗传研究表明，玉米抗丝黑穗病属数量性状遗传，受多基因控制。前期研究中我们精细定位了抗病主效QTL，克隆了相应的抗病基因ZmWAK，并对抗病机理进行了初步探讨。鉴于玉米ZmWAK蛋白属跨膜受体激酶，本研究将系统鉴定和解析ZmWAK膜外受体功能域识别的病原菌致病因子，明确与ZmWAK互作的膜蛋白和胞内蛋白，探讨抗病信号通路，揭示免疫反应的分子、细胞学机制，从而全面阐述玉米丝黑穗病数量抗性的分子基础。本申请项目将深化植物数量抗性的分子机制研究，为培育持久广谱的抗病品种提供理论依据。

Maize head smut is a soil-borne disease caused by the fungal pathogen Sporisorium reilianum. The disease occurs in most maize growing areas worldwide and poses a great threat to maize production. Genetic analysis indicates that maize resistance to head smut is a quantitative trait, controlled by multiple resistance genes. In our previous study, we have fine-mapped the major resistance QTL-qHSR1 and isolated the causal resistance gene ZmWAK. Furthermore, we have preliminary investigated the molecular mechanism underlying the ZmWAK-mediated head smut resistance. Given that ZmWAK is a transmembrane pattern recognition receptor kinase, we plan to systemically screen and characterize ZmWAK-perceived pathogenetic effectors, identify ZmWAK-interacting host membrane-anchored and intracellular proteins, investigate the disease resistance signaling pathways, and unveil the molecular and cytological mechanisms underlying the immune response. All these researches would allow a comprehensive understanding of the molecular basis of maize quantitative resistance to head smut. The study will go insight into the molecular mechanism of quantitative disease resistance in plant species and provide theoretical basis for development of broad-spectrum and endurable resistant varieties.