抑霉唑（Imazalil）和腐霉利（Procymidone）作为一类高效低毒的广谱型杀菌剂，是我国农业部推荐使用并确认具有抗雄激素活性的农药，且对果蔬的病虫害防治效果良好，因此至今仍在大量使用，在环境及果蔬中的大量残留对生态环境和食品安全均具有严重的潜在风险。而作为杀菌剂，抑霉唑和腐霉利主要的作用靶标是微生物，因此可能对肠道菌群产生影响，从而导致肠肝循环紊乱，最终引发为肠道及肝脏疾病。本研究拟通过饮水对妊娠期和哺乳期C57BL/6母鼠施以环境相关浓度的抑霉唑和腐霉利，通过小鼠生长表型、血液生化指标、病理切片、相关基因和蛋白水平、微生物丰度检测等来评估抑霉唑和腐霉利暴露对F0、F1或F2代小鼠肠道菌群、肠道屏障功能、肠肝循环、免疫以及内分泌系统产生的影响，研究抑霉唑和腐霉利暴露后对母鼠自身及生命早期和青春期的后代所造成的毒性效应。研究结果可为评价杀菌剂等农用化学污染物对特殊时期人体健康的影响提供证据。

Imazalil (IMZ) and procymidone (PRO) is a kind of broad-spectrum, efficient and low-toxicity fungicide. They are recommended by the Chinese ministry of agriculture for use in vegetables and fruits which is confirmed to have antiandrogenic activity. Due to the good effect of disease and insect pest control on fruits and vegetables, they are still widely used today. Their residues in the environment, fruits and vegetables have serious potential risks to the ecological environment and food safety. As a fungicide, IMZ and PRO target a wide range of microorganisms and thus may have detrimental effects on our gut microbiota, a complex microbial ecosystem formed by a large variety of microorganisms that had coevolved with the host. This can lead to enterohepatic circulation disorders, and eventually lead to intestinal and liver diseases. Here, IMZ and PRO were oral administered to C57BL/6 female mice at the level of environment-related concentration during the gestation and lactation periods in the F0 generation. Biochemical indicators, pathological section, related gene and protein levels, and microbial abundance tests were used to assess the effects of maternal IMZ and PRO exposure on F0, F1 or F2 mice, including gut microbiota, intestinal barrier function, enterohepatic circulation, immune system and endocrine system effects. The aim was to study the toxic effects of IMZ and PRO on dams and their early life and adolescent offsprings. The results can provide evidence for evaluating the effects of fungicides and other agricultural chemical pollutants on human health in special periods.