肿瘤相关基因表达的转录调控是影响肿瘤生物学功能的关键环节。在前期研究中，我们发现结肠癌中高表达的S100P受转录因子SOX9调控，通过激活RAGE/ERK通路，促进肿瘤的增殖与侵袭转移。同时，S100P除分布于结肠癌细胞细胞浆中，在细胞核内也有丰富的表达。为探索肿瘤细胞核内的S100P是否具备转录调控功能，我们通过ChIP-sequencing分析及筛选发现核内S100P可与POTEE启动子相结合。为进一步分析核内S100P转录调控POTEE在结肠癌增殖凋亡中所发挥的作用，本项目将通过构建S100P、POTEE干扰及过表达结肠癌细胞株，验证结肠癌细胞核内S100P的基因表达调控功能，阐述受核内S100P转录调控的POTEE基因在结肠癌增殖中的作用及其分子机制，阐明POTEE在S100P促结肠癌增殖中的意义与地位，补充及完善结肠癌中S100P分子作用理论，为对抗结肠癌的增殖提供新的治疗策略。

Transcriptional regulation of cancer-related gene expression is an important way to affect the tumor biological function, and a key step to explain the process of cancer development. In our previous study, we found that S100P was an overexpressed protein in colon cancer. High expression of S100P regulated by the transcription factor SOX9, activated RAGE/ERK pathway, promoted the proliferation, invasion and metastasis of colon cancer. And we also found that S100P was expressed in abundance in the nucleus, besides distributed in the cytoplasm of colon cancer cells. We used ChIP-sequencing to explore the function of intranuclear S100P in transcriptional regulation. It showed that intranuclear S100P could be combined with POTEE promoter. To analyse the role of POTEE transcriptional regulated by S100P in the colon cancer, this study will validate the relationship between S100P and POTEE, detect the effect of POTEE in proliferation and apoptosis of colon cancer cells, clarify the meaning of POTEE in S100P-mediated proliferation of colon cancer, and discover POTEE-related pathways by constructing S100P or POTEE interfered and overexpressed colon cancer cell lines. In the end, the gene expression regulating function of S100P in the nucleus would be verified. The biological function of POTEE and related mechanisms in the colon cancer would be clarified. The results of this study could enrich the molecular theories of S100P in colon cancer, and might offers a new strategy for targeted cancer therapy against the proliferation.