白藜芦醇（Res）有神经保护和修复作用，但机制未明限制其临床应用，因此，阐明其机制则是解决问题的关键。Shh信号和Sirt1参与调控脑缺血后损伤和修复。课题组前期已在国际上率先证明Res能通过激活Shh信号改善卒中后神经功能，但作用靶点亟待阐明。Res是Sirt1特异激动剂，那么，Res激活Shh信号是否通过Sirt1介导？亟待研究。因此，本课题采用RNA干扰、转染等技术以神经干细胞和MCAOI/R模型大鼠为研究对象，系统地从体内外探讨Res对Shh信号的影响及作用靶点，Shh信号和Sirt1的相互作用以及干扰Sirt1和Shh信号后，Res对Shh信号、Sirt1、神经保护和修复作用的影响。以从Shh信号和Sirt1相互作用这个全新的角度阐明Res可能通过Sirt1→Shh信号这一"通路"促进卒中后神经功能恢复的新途径，为其早日用于临床以及开发设计新药奠定实验基础。

Resveratrol has neuroprotevtive and reparative effects. But, it is the key of limitting its clinic application that its mechanism of neuroprotective and repair effects has not been illuminated. Shh signaling and Sirt1 take part in controlling injury and repair after ischemic stroke. Our previous study had firstly demonstrated that resveratrol could promote neural functional recovery via activating Shh signaling. However, its target point is unclear. In addition, many reports have showed that resveratrol is an activator of Sirt1. But, it is unclear whether Shh signaling is connected with Sirt1. Therefore, with a number of techniques such as RNAi and gene transfection systematically, we study effects and target point of resveratrol on Shh signaling, crosstalk of Shh signaling and Sirt1, and effects of resveratrol on the Shh signaling ，Sirt1，neuroprotectiveon and repair after disturbing Sirt1 and Shh signaling on MCAO I/R model rats in vivo and neural stem cells in vitro.Finally, from the new perspective of crosstalk of Shh signaling and Sirt1，we will expound a new way that resveratrol may passage the "pathway “ of the Sirt1 → Shh signals to promote functional recovery after stroke , which will provide firmly experimental basis toward the development of next generation therapeutics for resveratrol and new drugs.