慢性疼痛治疗的关键难题是疼痛发生传递的机制不明。脊髓背角是痛觉信息加工转换的重要节点，最新研究表明，脊髓背角内的囊泡膜谷氨酸转运体2（VGLUT2）是与躯体痛觉信息传递密切相关的神经递质标记物。前期临床和实验表明推拿具有较好的镇痛作用。我们假说推拿能通过下调VGLUT2的表达而起到阻断疼痛传递的作用。. 拟将96只大鼠随机分为正常组、手术组、模型组、假推拿组、慢病毒注射组和推拿组，后四组进行L5脊神经结扎，假推拿给予抚摸，慢病毒注射组给予注射能有效降低VGLUT2的shRNA重组慢病毒，推拿组给予推拿手法。实验第7、17d观测行为学及取材，免疫荧光组织化学染色法测各组大鼠脊髓神经元内绿色荧光蛋白的表达；免疫蛋白印迹技术检测VGLUT2蛋白的表达。探讨下调VGLUT2对痛觉信息传递影响的机制及推拿的干预效应，为推拿镇痛提供客观依据。

The key thorny-issue of treating chronic pain is the unknown mechanism of pain transmission. The spinal-doral-horn is an important node in the pain signals processing conversion. The latest research shows that the VGLUT2 within spinal-doral-horn is the neurotransmitter markers that is closely associated with body nociceptive information transmission. Previous clinic and experiment indicated that the massage has good analgesic effect. So we make the hypothesis that massage has the effect of blocking pain transmission by down-regulating the expression of VGLUT2.. 96 rats were randomly divided into normal group, model group, fake massage group, slow virus injection group and the massage group. The hinder four groups were given L5 spinal nerve ligation; false massage group was given dauting; slow virus injection group was given injecting shRNA recombinant virus which can effectively reduce VGLUT2; while the massage group was given massage. In 7, 17 day of experimental cycle, observed behavior and draw materials, then measured the expression of green fluorescent protein in spinal cord neurons of each group rats by using immunofluorescence histochemical staining method. Detected VGLUT2 protein expression by immune protein imprinting technology. To investigate the mechanism of down-regulation VGLUT2 on the influence of nociceptive information transmission and intervention effect of massage, then provide objective basis for massage analgesia.