人脂肪间充质干细胞（ADSCs）移植在各种帕金森病（PD）动物模型中都得到了有效验证，电针（EA）治疗PD临床疗效确切，但两者均缺少深入的机制研究。前期研究发现PD个体存在线粒体自噬和损伤，而PINK1/Parkin介导的自噬溶酶体通路是线粒体自噬的研究热点。我们推测EA、ADSCs移植以及两者联合可能通过此通路抑制线粒体自噬过度激活，缓解了线粒体结构和功能破坏。为验证此假说，本研究应用MPTP皮下注射制作老年恒河猴PD模型，经颈内动脉移植ADSCs，应用行为学评估、MRI、HPLC、电镜、ELISA以及免疫荧光双标等技术，探究内外源干细胞存活、增殖、分化、迁移和线粒体超微结构的变化；分析泛素接头蛋白P62与线粒体自噬相关蛋白（α-synuclein、Parkin、Ub、ERK1/2、LC3等）、线粒体定量酶CS、呼吸链复合体II、IV和COXI相互作用的调控机制，为治疗PD提供实验依据。

The therapeutic efficacy of human adipose-derived stem cells (ADSCs) has been assessed in various animal models with PD. The treatment of PD using electro acupuncture (EA) has long been also effective. Previous studies have found that individuals of PD have mitochondrial dysfunction and the mitophagy. Meanwhile, the mitophagy occurring through activated PINK1/Parkin pathway has long been the focus of many researchers. We proposed to test the hypothesis that EA and ADSCs may have therapeutic potential for PD by regulating the autophagy-lysosome pathway and inhibiting excessive activation of mitophagy and alleviating the damage of mitochondrial structure and function. Therefore, the aim of this study was to use the aging Monkey (Macaca rhesus) model of PD could be created by the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)injection into the subcutaneous. ADSCs is transplanted into the brain of the PD monkey model by bilateral carotid artery respectively. The behavior cold be observed before and after the injection of MPTP in all the animals by Kurlan Scale; the proliferation, differentiation, migration of exogenous and endogenous stem cell will be examined through immunohistochemical method or MRI. Also, metabolites of dopamine (DA)will be examined by high performance liquid chromatography (HPLC), and biochemical analysis of blood for the relevant indicators of oxidative stress level. The mitochondrial ultrastructural changes in dopaminergic neuron(DN) was observed by transmission electron microscope; the content of quantitative enzyme CS and the activities of mitochondrial respiratory chain complex II,IV and COXI were measured by ELISA; the protein expressions of DN mitochondrial α-synuclein,Parkin,Ub,ERK1/2 and LC3 were detected by western blot and their amount of co-localization of mitochondria respectively with Parkin,Ub,p62 and LC3 were measured by immunofluorescence double labeling technique. Overall, this research could be the pre-clinical experimental basis of ADSCs transplantation for the treatment of PD.