放射性唾液腺炎（RS）是影响头颈放疗后生存质量的重要因素，其患病率逐年上升且发病机制尚不明确。我们前期研究显示，TGF-β1信号通路参与了RS，但该信号通路在体内广泛存在，不能够作为预防该疾病的靶向位点。而前期研究发现含TGF-β1/TβR复合物的外泌体以及同唾液腺发育相关的lncRNA具有组织特异性且在病理状态下影响RS。因此，我们考虑在RS中TGF-β1及含TGF-β1/TβR复合物的外泌体可能通过上调发育相关lncRNA导致唾液腺炎症、纤维化及细胞凋亡。本研究拟使用体外器官培养筛选发育相关lncRNA，通过体内外研究探究TGF-β1、含TGF-β1/TβR复合物的外泌体及相关lncRNA同RS的关系；通过ChIP-seq、免疫共沉淀、DNA-seq、RNA-seq阐释机制。通过体内研究，构建APQ5-Cre;TβRIflox/flox小鼠进一步探讨相关作用机制，为防治RS提供依据。

Radioactive sialadenitis (RS) is one of the main factors that influences quality of life for head and neck cancer patients receiving radiotherapy. Its prevalence increases but its mechanism is still unknown. Our previous study revealed that TGF-β1 pathway was involved in RS, however, this pathway participated in multiple physiological process of human body and could not be recognized as a treatment target. But our other research also indicated that TGF-β1/TβR containing exosomes and development related lncRNAs had tissue specific expression and were involved in the pathological process of RA. So, we consider that TGF-β1 or TGF-β1/TβR containing exosomes could upregulate development related lncRNAs and cause fibrosis, inflammation and apoptosis in RS. To prove this, we’ll use in vitro organ culture to select related lncRNAs. And via in vivo and in vitro studies, we aim to exam the relationship among TGF-β1, TGF-β1/TβR containing exosomes, development related lncRNAs and RS. And we’ll use ChIP-seq, Co-IP, DNA-seq, RNA-seq to explain related mechanisms. And we’ll develop APQ5-Cre;TβRIflox/flox mice to further explain the mechanism to provide information for RS prevention and treatment.