我们前期的研究表明，前列腺素E2（PGE2）和17α,20β-双羟孕酮（DHP）是中华乌塘鳢（Bostrichthys sinensis）的主要性信息素，两者均能刺激性成熟中华乌塘鳢雌雄鱼的嗅上皮产生明显的嗅电反应、吸引性成熟雌雄鱼配对进入人工产卵巢以及诱发雌雄鱼的交配产卵行为。为了阐明中华乌塘鳢这两种性信息素作用的分子机理，本项目拟采用现代分子生物学等技术，开展以下研究内容：(1)嗅上皮中PGE2和DHP受体基因的克隆;(2)嗅上皮中PGE2和DHP受体的表达和功能验证;(3)嗅上皮中PGE2和DHP受体的定位;(4)性信息素对生殖内分泌系统的调控;（5）生殖内分泌激素对嗅上皮性信息素受体表达的反馈调控。研究结果可丰富鱼类化学通讯研究的基础理论，也可为海水鱼类诱导产卵技术的改进提供科学依据，具有重要的理论意义和潜在的应用价值。

Our previous studies showed that prostinglandin E2 (PGE2) and 17α,20β- dihydroxyprogesterone (DHP) are the chief sex pheromones in the Chinese black sleeper (Bostrichthys sinensis). These pheromones elicit rapid electro-olfactory responses, attract females and males to the artificial nests, and induce spawning behaviors in sexual mature fish. In order to elucidate the molecular mechanisms of these sex pheromones, using modern molecular and biochemical techniques, we will carry out the following studies: (1) cloning PGE2 and DHP receptor cDNAs in the olfactory epithelium; (2) expression and characterizing functions of PGE2 and DHP receptors in the olfactory epithelium; (3) localization of PGE2 and DHP receptors in the olfactory epithelium; (4) pheromonal regulation on the reproductive system; and (5) feedback regulation of reproductive hormone on the expression of the pheromonal receptors in the olfactory epithelium. The results from this study will contribute to the development of basic theory in the field of chemical communication in fish, and provide a scientific basis for the technical improvement of the spawning induction in fishes; therefore advance science and aquaculture applications.