PKR作为dsRNA依赖的、干扰素诱导的蛋白激酶参与多种细胞因子介导的抗病毒免疫反应和细胞凋亡。哺乳类PKR除了抗病毒作用，还具有广泛的生物学功能。近年来，包括草鱼PKR在内的多个鱼类PKR基因陆续被克隆和鉴定。对鱼类PKR的研究刚刚开始，多集中于抗病毒、对eIF2α磷酸化和诱导细胞凋亡等方面，而鱼类PKR如何被激活和启动免疫反应的机制没有涉及。申请人前期发现：1、草鱼PKR与其调控蛋白PACT和TARBP2可形成三聚体，而dsRNA能够使三聚体解体从而解放了PKR；2、草鱼PKR能够与IKKβ结合。为解析鱼类PKR活化及其激活NF-кB的分子机制，本研究拟通过研究草鱼TARBP2、PACT和PKR的互作，IKKβ与PKR、IкBα的互作，从PKR的磷酸化和二聚化了解PKR的活化机制；从IKKβ与IкBα的磷酸化以及p65/p50的互作与入核分析NF-кB信号通路的启动激活分子机制。

As the interferon-induced and dsRNA-dependent protein kinases, PKR is involved in a variety of cytokine-mediated antiviral immune responses and apoptosis. In mammal, PKR has a wide range of biological functions in addition to antiviral effect. In recent years, PKR has been cloned and identified in many fish species, including Grass carp PKR. The study of PKR in fish has only just begun. The researches focus on its antiviral activity, phosphorylation of eIF2α and induction of apoptosis. However, these researches did not involve how PKR is activated and how immune response is activated by PKR. In our previous study, we found grass carp PKR can form trimer with its regulatory proteins PACT and TARBP2. However, the trimer will be broken up by the induction of dsRNA, and then PKR will be liberated. Secondly, grass carp PKR can bind to IKKβ. To investigate the activation molecular mechanism of PKR and NF-кB, this study focuses on the interaction between the Grass carp TARBP2, PACT , PKR, the grass carp IKKβ and PKR, IкBα respectively. On the one hand, to understand the activation mechanism of the phosphorylation and dimerization of PKR, On the other hand, to analyze the activation and molecular mechanism of the NF-кB signaling pathway from the phosphorylation of IKK and IкBα, the interaction and transnucleation of p65/p50.