慢性肾衰竭营养不良（CRF-PEW）严重威胁公民健康，骨骼肌萎缩是其重要病理，目前我国尚无特异性药物。肌卫星细胞（MSC）是对骨骼肌萎缩具有修复作用的干细胞，在CRF状态下功能受到抑制。jagged1-notch信号通路参与多种干细胞激活和分化，但在CRF-PEW骨骼肌萎缩中的地位尚不明确。课题组通过前期研究发现，人参养荣汤能改善CRF患者PEW状态，且影响jagged1表达水平。因此我们推测jagged1-notch信号通路参与人参养荣汤治疗CRF-PEW的机制。本课题拟通过CRF-PEW大鼠和细胞模型，构建真核表达系统确定jagged1-notch通路在CRF-PEW中的作用机制；应用体内实验验证人参养荣汤对模型动物的表型影响；采用RNAi技术体外验证jagged1-notch信号在中药作用机制中的地位；探讨jagged1-notch通路在CRF-PEW中的作用及中药治疗的分子机制。

Chronic renal failure-Protein energy wasting(CRF-PEW)is a diseases which seriously harm human health.It’s important pathological manifestations is Skeletal muscle atrophy.There is no effective drug treatment of this disease at present.Muscle satellite cells(MSC) has a important repair effect to skeletal muscle atrophy,but this function is restrained in CRFstate. Jagged1-notch signaling pathways involved in a variety of stem cell activation and differentiation,but it’s role of CRF - PEW skeletal muscle atrophy is unclear. Our research by previous studies found that RenShenYangRongTang can improve the CRF-PEW status and affect the level of jagged1 expression, So we guess that jagged1 - notch signaling pathways involved in CRF-PEW mechanism by RenShenYangRongTang. This topic proposed by CRF PEW SD rats and cell model, construction of eukaryotic expression system to determine the role of jagged1-notch pathway in CRF-PEW; application in vivo experiment of animal models with RenShenYangRongTang to prove phenotypic effects;using RNAi technology in vitro validation to prove the role of jagged1-notch signaling in Chinese medicine;then research the role of Jagged1-notch pathway in CRF-PEWand the molecular mechanism of traditional Chinese medicine treatment.