寒地颞叶癫痫硬化海马差异基因神经元3D网络MRI图谱研究

寒地癫痫具有患病率较高、死亡风险较高、治疗缺口较大特点。尽管其中大部分为颞叶癫痫（TLE），是最佳手术适应症，但由于影像学尚不能精准显示TLE致痫3D网络和发作放电传导路径，导致近1/3患者无法治愈，迫切需要针对TLE硬化海马分子影像做出精准评估。本研究以寒地TLE硬化海马为研究对象， 基于9.4T磁共振成像（MRI），制作硬化海马MRI模板；采用单细胞测序技术，构建硬化海马细胞分类图谱和染色质可及性图谱，绘制差异基因调控网络和标志基因调控网络；采用组织光透明成像技术，制作硬化海马神经元和差异基因神经元网络图谱；对3D网络进行智能配准，构建介观层次的差异基因神经元3D网络MRI图谱；采用立体脑电图技术，绘制硬化海马致痫电极网络图谱；应用多模态多复杂网络关联分析法，构建TLE发作放电传导路径图谱。本研究通过医工结合，多学科协作，提高MRI分辨率，实现致痫网络可视化。上述图谱目前未见报道。

Cold region epilepsy is featured with high morbidity, high mortality risk and lack of treatment. Although temporal lobe epilepsy (TLE) occurred most and counted as the best indication for surgery, TLE epileptic 3D networks and electrical conduction pathways of seizures cannot be visualized precisely in imaging, which lead to one third of the patients incurable. Precision evaluation of TLE hippocampal sclerosis in molecular imaging is needed urgently. In this research, we intend to study sclerotic hippocampi of TLE patients who live in cold region. Sclerotic hippocampi will be scanned with 9.4 T magnetic resonance imaging (MRI) to generate MRI template of sclerotic hippocampus. Single cell sequencing technology will be utilized to construct neuronal classification atlas and atlas of chromatin accessibility, delineate neuronal differential gene regulatory networks and marker gene regulatory networks of sclerotic hippocampus. The technology of tissue optical clearing imaging will be applied to generate neuron network atlas and differential gene expressed neuron network atlas of sclerotic hippocampus. Differential gene expressed neuronal 3D network MRI atlas will be constructed at mesoscopic level with intelligent spatial registration for 3D networks. In addition, technology of stereoelectroencephalogram will be utilized to delineate epileptic electrode networks of sclerotic hippocampus. Correlation analysis for multimodal complex networks will be adopted as well to construct electrical conduction pathways of TLE seizures. Our study roots on the principle of medical-industrial integration and multidisciplinary collaboration to help enhance the visualization of the epileptic networks on MRI by increasing MRI resolution at molecular level. There is no report on the aforementioned atlases.