由稻瘟菌菌引起的稻瘟病严重威胁世界粮食安全，稻瘟病菌致病机制的研究将为设计高效特异防治稻瘟病的农药提供强有力的技术支撑和保障。海藻糖-6-磷酸合成酶（TPS）MoTps1是稻瘟病菌致病所必需的，它不仅是海藻糖合成的关键酶，还通过感知G6P和与NADPH结合调控碳氮代谢等。为分析MoTps1的致病机制及其与水稻OsTPS1和细菌OtsA在结构和生化机制的异同, 本研究拟综合运用生物化学和生物物理学手段制备各个TPS蛋白；分析其与底物等配体的结合强度和比例；解析MoTps1和OsTPS1自身及其配体复合物的三维结构，建立它们结合配体的模式；分析不同TPS结合配体后的变化动态和参与相互作用界面等的差异以及基于差异区域开展特异性抑制剂的筛选。上述结果将是分析不同物种TPS功能差异分子机制重要的结构基础，还为筛选MoTps1特异性的小分子抑制剂以设计和开发环境友好型高效特异性杀菌剂奠定坚实的基础。

The rice blast disease caused by Magnaporthe oryzae is a serious threat to world food security, and research on the pathogenic mechanism of M. oryzae will provide strong support for the design of high effective pesticides against it. MoTps1, the trehalose-6-phosphate synthase (TPS) in M. oryzae, is essential for the pathogenicity of the fungus. It is not only a key enzyme for trehalose synthesis, but also involves in the regulation of carbon and nitrogen metabolism by sensing G6P and binding to NADPH. In order to analyze the pathogenic mechanism of MoTps1 and the differences in structures and biochemical mechanisms of MoTps1, OsTPS1 and OtsA, we will purify these TPS proteins and measure the interaction parameters between these proteins and ligands, such as catalytic substrates, NADPH and so on. Meanwhile, the three-dimensional structures of MoTps1, OsTPS1 and their complexes with ligands will be determined by structural biological approaches, and the differences on the patterns of ligand binding, dynamics during ligand binding and the surface characteristics will be analyzed based on the solved structures. Then the specific inhibitors for MoTps1 will be screened virtually on the regions investigated above. These differences are not only the main structural basis of functional differences of TPSs in different species, but also provide a solid basis for virtual screening for specific inhibitors against MoTps1 and further for the design of environmentally friendly fungicides with high efficiency.