基于NRGl/ErbB4信号紊乱在“靳三针”治疗孤独症中作用机制的研究

孤独症谱系障碍（ASD）是一种病因不明的严重神经发育障碍，发病率上升明显。功能研究发现孤独症与突触发育、突触连接功能异常相关。其中NRG1/ErbB4 信号转导调控神经发育和突触可塑性，影响多巴胺、谷氨酸和γ氨基丁酸等多个神经递质系统，与孤独症密切相关。临床上证实“靳三针”治疗孤独症有效，国家中医药管理局已将"靳三针治疗儿童精神发育迟滞和儿童自闭症临床规范化研究"两项成果作为临床适宜疗法向全国推广，对其作用机理的基础研究成为热点。本研究拟以NRG1/ErbB4 信号通路为切入点，通过 “靳三针”针刺孤独症大鼠模型以及目标基因胚胎电转模型的额顶区（智三针、四神针），进一步分析在“靳三针”作用下，NRG1/ErbB4 信号转导变化引起的分子、细胞及整体动物行为发生的变化，为针刺治疗孤独症的作用机制提供分子生物学基础。

Autism Spectrum Disorder (ASD) is a serious neurodevelopmental disorders of unknown etiology with a significant increase in morbidity. Functional studies have found that autism is associated with abnormal synaptic development and synaptic connections. Among them, the NRG1 / Erb B4 signal transduction regulates the neurodevelopment and synaptic plasticity ,and affects many neurotransmitter systems such as dopamine, glutamic acid and γ-aminobutyric acid, which are closely related to autism. Clinically proved that Jin’ 3-needles have effective treatment in autism,the State Administration of Traditional Chinese Medicine has two results as a clinical appropriate therapy that push to the Nationwide promotion,called"Jin’ 3-needles clinical treatment and research of of children's mental retardation and children's autism" . The basic research on its mechanism has become a hot topic. In this study, NRG1 / ErbB4 signaling pathway as the starting point, through the acupuncture autism rat model and the target gene embryonic transfer model of the tophead/forehead area (zhisanzhen acu-point,sishenzhen acu-point).And Further analysis of changes in molecular, cell and overall animal behavior caused by NRG1/Erb B4 signal transduction under the action of "Jin’ 3-needles",which could provide a basis for the molecular mechanism of acupuncture in the treatment of autism.