当代谢葡萄糖供给量较大时，动物乳腺糖酵解增强并产生大量乳酸，从而损害乳腺健康并降低乳腺合成能力。目前，泌乳动物乳腺糖酵解的研究越来越受到关注，但其调节机制相关研究较少。碳水化合物反应元件结合蛋白(ChREBP)是营养素调节动物乳腺糖酵解的重要靶基因，因此明确ChREBP介导的乳腺糖酵解调节机制对维护乳腺健康和改善泌乳效率具有重要意义。本项目拟结合我们前期工作基础，以奶山羊乳腺上皮细胞为模型，研究ChREBP对营养素的动态应答及其对乳腺糖酵解活力的影响，探究过表达或干扰ChREBP对乳腺糖酵解相关信号网络、代谢图谱和酶活的影响。通过这些研究，在细胞水平上揭示ChREBP调节奶山羊乳腺糖酵解的机制，为通过营养手段调控反刍动物乳腺健康提供基础数据。

When large quantity of metabolic glucose is supplied to lactating animals, the glycolysis in the mammary gland (MG) is enhanced and lactic acid is largely produced, which finally damages the MG health and reduces the efficiency of milk production. Recently, the glycolysis in the lactating MG has drawn great interest, but the literature is limited on the regulatory mechanisms. The carbohydrate responsive element binding protein (ChREBP) is an important target for the glycolysis in the MG. Thus, it is important to clarify how ChREBP regulates the glycolysis for the MG health and efficiency of milk production. The proposed project is intended to study the effects of glucose on expression of ChREBP and the glycolysis levels, and to investigate the effects of overexpression/silencing of ChREBP on functional network of glycolysis and on metabolites and enzyme activities related with glycolysis in the MG. By conducting the above-mentioned work, our study attempts to reveal the role and mechanism of ChREBP in regulating the MG glycolysis, which will contribute to the nutritional manipulation of the ruminant MG health.