转移是导致肝癌恶性进展的重要因素，找到影响转移的关键分子尤为重要。糖核核酸酶 L（RNase L）是RNA内切酶，通过剪切内源或外源RNA发挥作用。RNase L可抑制能诱发肝癌的HBV 及HCV，但是否影响肝癌转移未见报道。课题组前期研究发现：RNase L在肝癌组织表达明显低于癌旁，可抑制Huh7等细胞转移，影响转移相关lncRNA-ATB表达。结合文献RNase L的抑癌基因作用及前期结果，提出假设：1. RNase L在体内外可抑制肝癌细胞转移；2. RNase L 可通过调节lncRNA-ATB通路抑制肝癌转移。本项目拟通过细胞侵袭转移、细胞黏附、血管形成及裸鼠成瘤等实验研究RNase L对肝癌细胞转移的抑制作用；通过共转染、RIP、RNA半衰期及RNA体外转录加工等实验研究RNase L通过lncRNA-ATB抑制转移的机制。本研究将为肝癌的靶向治疗提供新靶点及理论依据。

Metastasis is an important factor leading to malignant progression of liver cancer. It is important to find the key molecules that affect the metastasis of hepatocellular carcinoma. Endoribonuclease L（RNase L) is an enzyme of RNA, which plays an important role through cleavage of endogenous or exogenous RNA. RNase L can inhibit the inducing factors（HBV and HCV）of liver cancer, but it has not been reported the effect of RNase L on metastasis of liver cancer. The previous study showed that the expression of RNase L in HCC tissues was significantly lower than that in the adjacent tumor, RNase L could inhibit Huh7 and other cell migration, and influence the expression of lncRNA-ATB. Combined with the role of RNase L as anti-oncogene and the previous results , we put forward the hypothesis that : 1.RNase L can inhibit the metastasis of hepatocellular carcinoma cells in vitro and in vivo; 2. the inhibitory effect of RNase L on the metastasis of liver cancer by regulating the lncRNA-ATB pathway. This project through the cell invasion and metastasis, cell adhesion, angiogenesis and inhibition of tumorigenicity experiments to confirm the inhibition of RNase L on metastasis of hepatocellular carcinoma cells; RIP, RNA half-life and RNA cleavage assay in vitro are used to confirm the molecular mechanism of RNase L regulating lncRNA-ATB . The project will provide new targets and theoretical basis for targeted therapy of liver cancer.