研究证实骨髓间充质干细胞（hMSC）具有修复受损肝组织和抑制肝癌生长的能力，且影响肝癌的转移潜能，使转移潜能下调。本研究目的是利用影像技术观察经TGFβ1和OPN基因修饰的hMSC移植前后肝癌转移潜能随时间变化的规律。磁性纳米颗粒SPIO标记hMSC，MRI追踪hMSC在肝癌动物模型体内的迁移、定位和分布。采用肿瘤转移黏附因子整合素αvβ3的特异性配体RGD多肽，分别行Gd和F-18标记，应用MR和microPET/CT对高低转移潜能肝癌动物模型进行成像。应用分子生物学技术分析hMSC影响肝癌转移潜能的信号通路关键蛋白酶B、TGFβ1、OPN与整合素αvβ3受体等生物学因子表达随时间变化与影像表现的关系的相关机制。本项目通过现代分子影像学手段在体、可视观察hMSC治疗前后肝癌转移潜能随时间变化的研究，解决转基因hMSC治疗肝癌效能及干预前后转移潜能变化的在体影像学实时监测的科学问题。

Studies have confirmed that bone marrow mesenchymal stem cells (hMSC) can repair the damaged liver tissue and have ability to inhibit the growth of HCC,and impact metastatic potential of HCC, and make metastatic potential downregulated. The purpose of this study is to observe the metastatic potential variation over time of HCC by TGF β1 and OPN gene modified hMSC transplantation using imaging technology. hMSC was labeled by magnetic nanoparticles SPIO, and MRI track hMSC migration, positioning and distribution in vivo. Tumor metastasis adhesion molecule integrin αvβ3-specific RGD peptide ligand was labeled respectively by Gd and F-18, then using new probes to observe the high and low metastatic potential animal models of HCC by MR and microPET / CT scanning. An analysis of the relationship and mechanisms between the metastatic potential of signaling pathway critical protease B, TGFβ1, OPN and integrin αvβ3 receptor expression and other biological factors and molecular imaging findings by molecular biology techniques. The project was the observation of metastatic potential changes of gene modified hMSC intervention HCC by means of modern molecular imaging,and to solve the imaging real-time monitoring scientific issues of the performance of gene modified hMSC intervention HCC and the metastatic potential changes in vivo.