针对癫痫发生的相关研究一直备受关注。前期工作中，我们通过建立颞叶癫痫模型，早期干扰脑内内源性大麻素2-AG的代谢，发现其能减少动物的慢性自发性发作，同时在一定程度上改善其情感及认知等行为学的变化，这种潜在的抗癫痫发生效果可能与早期的神经保护有关。鉴于给药途径的影响，本研究拟采用MRI监测下低频聚焦超声联合微泡造影剂的超声诊断学方法，利用桥接亲和素-生物素法制成运载载体，无创、有效地开放血脑屏障，将药物安全、靶向送至相应脑区，达到实时监控并治疗的目的，同时从神经保护、纤维芽生、炎症通路及与星形胶质细胞上内源性大麻素系统间的潜在作用等方面细化研究作用机理。本研究旨在利用一种新颖的透过血脑屏障的方法，探索内源性大麻素系统与癫痫发生间的序贯作用，为癫痫发生的实验研究及临床治疗提供理论依据。

Epileptogenesis refers to a dynamic process by which normal brain tissue is transformed into tissue capable of generating spontaneous recurrent seizures. The current treatment of epilepsy by antiepileptic drugs (AEDs) has been developed for symptomatic suppression of seizures and not for prevention of epileptogenesis. We evaluated whether early interferes with 2-AG metabolism after a pilocarpine-induced SE could abolish spontaneous epileptic seizures in adult male mice. Meanwhile, we investigated its effect on animals' behavior for sake of exploring the role of endocannabinoid system in epileptogenesis. The results indicate that timely decrease the level of 2-AG which seems to be antiepileptogenesis after SE could retard the progress of epilepsy, which may be attributed to the neuroprotective function against acute injure. Focused ultrasound combined with microbubbles (MBs) using appropriate parameters has been demonstrated to be able to open the BBB locally and noninvasively. This study will investigate the targeted delivery of RHC-MBs through the BBB by magnetic resonance imaging (MRI)-guided focused ultrasound during the period of epileptogenesis. In addition, the therapeutic time window and possible molecular mechanisms underlying this process could also be identified. The present study will provide an insight into the potential relevance of endocannabinoid system and epileptogenesis by a potential effective method of Ultrasound imaging technique.