lncRNA-9130024F11Rik与基因Satb2介导的ceRNA在小鼠大脑皮层神经干细胞发育中的调控网络

小鼠大脑皮层的发育依赖非编码RNA（ncRNAs）与基因之间精细而复杂的调控。竞争性内源RNA (ceRNA) 是一类能够竞争性结合miRNA对靶基因调控的RNA分子，它涉及更多种RNA之间的调控网络。ceRNA的机制研究有助于更加深入、全面地了解大脑发育的调控。课题组前期研究发现，LncRNA 9130024F11Rik在小鼠大脑中特异性表达，与Satb2呈反向转录且部分重叠，提示该lncRNA可能存在与人Satb2-AS1类似的调控机制。本项目建立在9130024F11Rik敲除鼠的基础上，探索该lncRNA 与Stab2在神经干细胞分化中的相互调控机制。并通过全转录测序，初步构建9130024F11Rik与Satb2介导的ceRNA调控网络。本项目的顺利进行有助于从更深层面上挖掘lncRNA在神经干细胞中的功能和调控机制，并为开发大脑发育异常的ceRNA分子检测提供可靠的科研依据。

In the human and mouse cerebral cortex, differentiation of neural stem cells and progenitors relies on precise regulation correlation between gene and noncoding RNAs. Competing endogenous RNAs (ceRNAs) are transcripts that can regulate each other at post-transcription level by competing for shared miRNAs. CeRNA networks link the function of protein-coding mRNAs with that of noncoding RNAs such as microRNA, long noncoding RNA, pseudogenic RNA and circular RNA. CeRNA network analysis can help us to understand the precise regulatory mechanism of cerebral cortex development.In our preliminary studies, we have screened a lncRNA 9130024F11Rik that is highly expressed in the mouse embryonic cortex, and successfully obtained the 9130024F11Rik knockout mice. Based on previous results, in this project, we will first attempt to explore the roles of 9130024F11Rik in neural progenitor development. We speculate that 9130024F11Rik may regulate Satb2 by binding to its promoter sites, which is one of the possible regulatory pathway of cortical neural progenitor development. We further validate were used to identify Satb2-9130024F11Rik ceRNA network. The success of this project will provide crucial guidelines and technical supports for further investigating functions of lncRNAs, and understanding lncRNA mediated ceRNAs of neurological diseases.