复方叶下珠对URG11介导HBV相关HCC的β-catenin/Wnt作用

早期临床研究发现：复方叶下珠能减少血清中URG19和URG11阳性率，防止乙肝相关肝癌的发生。并在实验室证实了叶下珠能抑制URG19表达，但对URG11致癌的机理尚不清楚。弄清其机理是为了了解复方叶下珠是否具有直接抑制肿瘤细胞增殖的作用。基于URG11激活β-Catenin/Wnt信号的事实，提出了复方叶下珠能抑制URG11及其β-Catenin/Wnt信号作用的假说。首先建立HBx、URG11转染HepG2细胞模型，采用Northern、Western bolot、pTOPFLASH、Powerblot方法等，以瞬间共转染siRNA为对照，在体内外检测URG11及激活β-Catenin/Wnt信号。其次基于肝癌的关键病机虚、瘀，湿毒，设计的补气化瘀复方叶下珠对这一致癌途径干预的研究。本项目充实了HBxAg介导的相关肝癌机理的研究，探究了复方中药早期且多途径的防治肝癌作用。

Early clinical studies find that Compound Phyllanthus Urinaria L (CPUL) can prevented HBV related with hepatocarcinogenesis, reduced serum URG19 and URG11 positive rate. Experiments verification of CPUL can inhibited the expression of URG19 in vivo and in vitro, but URG11 efect hepatocellular carcinogenic mechanism, it is not clear? This aim is to determine CPUL whether to direct inhibit tumor cell proliferation. Base on URG11 carcinogenic research that initate the classic Wnt/beta-catenin signaling, put forward a hypothesis that CPUL may also inhibit expression of URG11 and β-Catenin/Wnt signaling. First establish HBx, URG11 transfection HepG2 cell model, and used mothed of the Northern blot, Western blot, immunoprecipitation,pTOPFLASH, Powerblot analysis to detect the expression of URG11, and the related effector of signaling,comparison with transfected siRNA .Secondly based on the main pathogenesis of HCC on traditional Chinese medicine (TCM) that are deficiency of blood ,blood stasis, poison and wet, set up CPUL that restore vital energy and Blood act stasis remove, intervent expression of URG11 and Wnt/β-catenin signaling in cell lines and nude mice. This studies that enriched the HBxAg mediated related HCC carcinogenic pathogenesis, and probe into the compound TCM has early cancer prevention and antitumor effect of multipe target.