基于Pan-genome技术的沙门氏菌血清型特异性基因挖掘、功能分析及分子鉴定

沙门氏菌(Salmonella)是最常见的食源性致病菌之一，用血清型来区分沙门氏菌是评估其危害等级的基础。本课题组前期研究发现沙门氏菌种特异性基因与决定血清型的抗原相关，但沙门氏菌血清型的特异性基因和其抗原的相关性仍不清楚。为此，本项目首先利用Pan-genome技术对沙门氏菌血清型基因组进行分析，挖掘出血清型特异性基因；利用InterProScan、KEGG等数据库和生物信息学工具分析血清型特异性基因的结构和功能，预测其在沙门氏菌抗原生物合成中的作用，揭示沙门氏菌血清型抗原代谢网络的演化规律；建立靶向血清型特异性基因的沙门氏菌血清型PCR鉴定方法，并对PCR方法进行特异性评价、体系优化和实际样品检测。本研究旨在阐释沙门氏菌血清型抗原与血清型特异性基因的相关性，并构建沙门氏菌血清型分子鉴定方法，为沙门氏菌流行病学研究、食品安全风险评估与预警提供理论指导和技术支持。

Salmonella is one of the most common food-borne pathogens. The serotyping to differentiate salmonella is fundamental to assessing their hazard classes. On the basis of the previous investigation on Salmonella enterica, it was observed that some species-specific genes were relevant to the antigens determining the Salmonella serotype.However the correlation between serotype-specific gene and the Salmonella serotype remains unclear. Therefore, we will firstly mine serotype-specific gene of Salmonella enterica using Pan-genome technology. To reveal the metabolism network of the antigen of Salmonella serotype, the structure and function of serotype-specific genes will be analyzed by the InterProScan,KEGG and other bioinformatics tools，and its role in the biosynthesis of the antigen will be also analyzed. PCR methods targeting serotype-specific genes will be established and optimized for the identification of Salmonella serotype. And these PCR methods will be evaluated by their specificity and the detection of actual samples. In a word, the project aims to elucidate the relationship between the antigen and serotype-specific gene in Salmonella serotype, and further construct the molecular identification methods of Salmonella serotypes, which will provide theoretical guidance and technical support for epidemiology research of Salmonella, risk assessment and early warning of food safety.