周围神经损伤后，骨骼肌因为失去神经营养作用而发生不可逆性损伤萎缩。保持失神经支配骨骼肌卫星细胞的再生活性，是失神经支配骨骼肌有效功能恢复的关键。研究发现，Wnt/β-catenin信号通路在骨骼肌损伤修复中起着非常重要的作用，激活Wnt/β-catenin信号通路传导可以促进失神经支配骨骼肌再生修复。我们前期研究显示：推拿通过改善失神经支配骨骼肌内的微环境以及骨骼肌内残存神经的活性和分泌能力，可以延缓失神经骨骼肌的萎缩，但这是否和推拿手法激活Wnt/β-catenin信号通路传导有关，具体的作用机理仍不甚清楚。因此本项目拟通过观察失神经支配后骨骼肌中Axin、GSK-3β的表达与运动终板结构与活性的变化、肌卫星细胞表达活性，阐明手法促进肌卫星细胞成肌分化的Wnt/β-catenin信号通路的调控作用，阐释推拿对失神经支配骨骼肌萎缩再生修复的作用靶点与途径。

After peripheral nerve injury,skeletal muscle irreversible atrophy because of the loss of neurotrophic effect.Keepinp the regenerative activity of muscle satellite cell Is the key of denervated skeletal muscle function recovery effectively.The study found that the Wnt/β-catenin pathway in skeletal muscle denervation muscle atrophy play a very important role. To activate Wnt/β-catenin pathway can retard denervated muscle atrophy.But the clinical researches in this area have not been seen.Our previous studies have shown that manipulation therapy can retard denervated skeletal muscle atrophy by improving the microenvironment of denervated skeletal muscle and the activity of remaining nerve.The reason may be manipulation therapy could activate the Wnt/β-catenin pathway. but the specific mechanism of action was still unclear.So intend of this project was to establish varying degrees of denervated rabbit skeletal muscle model firstly. The expression of Axin and GSK-3 beta in skeletal muscle and the changes in the structure and activity of the motor endplate and the expression of the muscle satellite cells were observed.Then to study regulation of manipulation therapy on Wnt/β-catenin pathway,to observe the target and the way of manipulation therapy in delaying denervated skeletal muscle atrophy.