获能是精子完成受精过程的一个必需条件，而精子表面去获能因子的释放是触发这一过程的关键。获能精子与去获能因子再次接触,又可呈现非获能状态。获能与去获能的可逆过程，提示去获能因子在这一对生理过程中起着重要的作用。申请人近年来对猪精子的功能及体外保存进行了一些研究，对猪精子体外保存时形态结构及功能的变化有一定的了解。为了深化前期的研究，本课题以广西地方猪种陆川猪精子为材料，以去获能蛋白为切入点，通过分离鉴定获能时精子表面释放的去获能蛋白，并用PCR、western blot、GST pull-down、质谱等多种技术，从分子、蛋白水平和信号通路角度研究其抑制获能的作用机制，试图发现新的去获能蛋白。同时，利用蛋白互作原理鉴定与去获能蛋白互作的精子膜蛋白，为阐明获能与去获能的可逆过程提供依据。研究结果可能对猪体外受精技术的完善和猪胚胎生产的商业化进程产生影响，为地方猪种的保存和改良提供理论基础。

It is indispensable for sperm to capacitate before undergoing the process to naturally fertilize the oocyte and this process is believed to be initiated following the release of surface-associated decapacitation factors. Moreover,capacitated sperm will reversibly lose their capacitated state when mixed with decapacitation factors. The reversible process between capacitation and decapacitation promprs that decapacitation factors play the role of a fatal bridge in this pair of progresses. In recent years, the apllicant has conducted a series of researches on the function and in vitro preservation of boar sperm, preliminarily understanding the change in morphology, structure and function of in vitro preserved sperm. To deepen the preliminary study, this project will be designed to discover decapacitation proteins and be expected to probe into the potential mechanism of capacitation from a perspective of decapacitation protein functions via separating/identifying decapacitation proteins released from capacitated sperm and on a molecular and protein level, from the standpoint of signal pathway, researching into the mechanism of their inhibition effect on capacitation by PCR, western blot, GST pull-down, and MS, using Luchuan pig (a local breed in Guangxi) spermatozoa as the treated samples and choosing decapacitation proteins as the breakthrough point. Furthermore, the theory of protein interaction will be practised to find sperm menbrane proteins which interacted with decapacitation proteins and provide a reference for the elucidation of the reversible process between capacitation and decapacitation. The results maybe impact the improvement of pig IVF and the commercialization of pig embryo production, thereby providing a theoretic reference for the conservation and improvement of a local pig breed.