绿盲蝽丁酸酯类性信息素降解酶的鉴定及在成虫求偶通讯中的功能分化研究

性信息素的灵敏特异识别对于昆虫求偶通讯至关重要。在嗅觉信号传递的分子通路中，受体被激活后，性信息素降解酶对剩余信号分子的清除有助于快速恢复受体神经元的敏感性。目前，鳞翅目蛾类昆虫性信息素降解酶的研究较为系统，半翅目盲蝽丁酸酯类性信息素降解酶研究十分匮乏。本项目拟以半翅目重要经济作物害虫绿盲蝽为研究对象，基于前期触角酯酶鉴定与进化分析的研究基础，拟先利用荧光定量PCR分析触角酯酶的表达谱。其次，通过原位杂交与免疫组化研究触角酯酶在绿盲蝽嗅觉感器中的表达定位。最后，通过体外酶动力学及活体水平RNAi系统的研究鉴定绿盲蝽丁酸酯类性信息素降解酶BPDEs并解析BPDEs在绿盲蝽求偶通讯中的功能分化。研究预期结果一方面有助于阐明半翅目盲蝽丁酸酯类性信息素降解的分子机理，同时也为探索建立BPDEs靶向的盲蝽RNAi体系，研发相应的害虫绿色防控技术提供科学依据。

The sensitivity and specificity of sex pheromone recognition is crucial for insect courtship behaviors. When pheromone receptor is activated, redundant sex pheromones in the sensillum lymph need to be degraded, in which pheromone-degrading enzymes (PDEs) play key roles help recover sensory neuron sensitivity. To date, the functional studies on PDEs of hemipteran mirids remain unclear in comparison to the well-known information of PDEs in lepidopteran moths. In the current proposal, we mainly focus on PDEs of Apolygus lucorum (Meyer-Dür), one of the most serious mirid pests of multiple economic crops, based on our previously succefully identification and phylogenetic analyses of antennal carboxylesterases (AlucCXEs). First, quantitative real-time PCR experiment is used to determine the AlucCXEs’ expression profiles. Then, in situ hybridizations and immunocytochemistry labeling are employed to examine AlucCXEs’ cellular expressions among A. lucorum different antennal sensilla. Finally, the vitro enzyme activities and kinetic estimations in combination with RNA interference (RNAi) in vivo are performed to investigate substrate spectrum and functional divergence of AlucCXEs in degrading the A. lucorum butyrate sex pheromones. The expected results would identify the butyrate sex pheromone-degrading enzymes (BPDEs) and clarify the molecular mechanism of butyrate sex pheromone degradation in hemipteran mirids, which in turn help develop the new environmentally friendly pest-management strategies for regulation of A. lucorum courtship by using BPDEs as new target in RNAi experiment.