井冈霉素是一种低毒生物杀菌剂，是我国防治水稻纹枯病的主打药剂、生产上往往频繁多次使用。我们在国内外首次发现井冈霉素刺激褐飞虱生殖现象，而且叶面喷雾处理的刺激效应大于点滴处理，但刺激生殖的机制至今不清楚。本项目采用双向电泳、LC-MS/MS及de-novo测序技术进行蛋白质组、转录组分析，筛选出与刺激生殖相关的关键基因；然后进行RNAi试验，通过qRT-PCR对RNAi效果验证；通过Kegg 和Pathway方法分析后，对相关基因用免疫共沉淀技术研究基因互作。目标是要阐明井冈霉素这个特殊药剂刺激褐飞虱生殖的分子机制。本项目将对发展害虫再猖獗理论、科学利用井冈霉在防治病害中的积极作用，避免其可能导致褐飞虱再猖獗发生的消极作用等方面具有重要意义。项目的特色和创新点为：研究材料（井冈霉素）特色鲜明，在国内外属空白研究；同时从脂肪体和卵巢的蛋白质组及转录组分析关键基因及其刺激生殖功能在国内外也属首次

Jinggangmycin is a conventional biofungicide with low toxicity which control rice sheath blight in China. The pesticide is commonly overused due to its low toxicity and high efficiency. Our investigation demonstrated that jinggangmycin stimulated reproduction of the brown planthopper(BPH) Nilaparvata lugens and that stimulatory effect of foliar spray with the jinggangmycin on reproduction of BPH was greater than that of topical application. However, molecular mechanisms of jinggangmycin-induced stimulation of reproduction is not understood. The present project will conduct proteome and transcription analysis on BPH treated with jinggangmycin by two-dimensional gel electrophoresis(2-D)and liquid chromatography tandem mass spectrometry (LC-MS/MS) and de-novo sequencing.Key genes responsible for stimulation of reproduction were selected and RNAi experiment to the genes will be conducted, finally, the efficiency of RNAi will be verified by qRT-PCR. Gene interactions are investigated by co-immunoprecipitation after analysis of Kegg and Pathway. The objective of the present study is to understand molecular mechanisms of jinggangmycin-induced stimulation of reproduction of BPH. The findings of the present study have important significance for development of pest resurgent theory for use of positive effect of jinggangmycin against rice sheath blight and avoidance of negative effect of jinggangmycin-induced stimulation of reproduction of BPH. The characteristic and innovative aspacts of this project are as follows: jinggangmycin-induced stimulation of reproduction of BPH is a gap study in pest resurgence in worldwide. In addition, protoem and transciption analysis from fat bodies and ovaries of adult females treated with jinggangmycin and selections of key genes associted with stimulation of reproduction based on the analysis and the determination of their finctions are a original innovative study in worldwide.