基于生物相分离与循环相结合的ABR-MBR一体化工艺反硝化除磷耦合调控机制研究

本研究将高效ABR与具有高截留分离效能的MBR工艺的有机组合，构建基于生物相分离和循环的、以优质内碳源提供和亚硝化为途径的多功能耦合联动的反硝化除磷一体化新工艺。研究中充分利用常规和聚合酶链式反应-变性梯度凝胶电泳（PCR-DGGE）和荧光原位杂交（FISH）等技术深入开展：（1）优质碳源提供及其有效利用的机制研究、生物相分离与循环微生物种群生物协同机制研究；（2）优势微生物菌群数量动态变化规律研究；（3）MBR亚硝化和ABR反硝化除磷耦合联动的关键机制研究，以获得实现稳定高效亚硝化和反硝化除磷的运行机制、系统中生物相分离与反硝化除磷菌对底物利用的生物协同机制，并建立优质碳源提供、亚硝化和反硝化除磷等过程的耦合联动机制，构建基于生物相分离条件下动态循环耦合系统运行的关键技术条件。

A combined process of high efficient Anaerobic Baffled Reactor(ABR) with Membrane biological Reactor(MBR) which is of high efficient biomass retention ability as a new multi-functional coupling integrated process is proposed here for denitrifying phosphorus removal based on the microbial phase seperation and circulation and as a way of inner carbon supply and Nitrosation. A series conventional and advanced microbiologial analytical methods such as the Denaturing Gradient Gel Electrophoresis ( PCR-DGGE ) and Fluorescence In Situ Hybridization ( FISH ) will be applied for the following study, including: (1)the mechanism of high quality C-source supply and its effective utilization, (2) synergu mechanism of biomass phase separation and circulation, the dynamic profile of dominant biomass population, and (3) the key coupling mechanism of between Nitrosation in MBR and denitrifying phosphorus removal in ABR, in order to reveal the biological synergy mechanism between bio-phase separation, Nitrosation and utilization of denitrifying phosphorus removal bacteria(DPB) for obtaining high efficient and stable denitrifying phosphorus removal, and finally, the key operational tactics and dynamic control model for coupling high quality C-source supply, Nitrosation and denitrifying phosphorus removal is aimed to be proposed.