钙粘蛋白是细胞间信号转导、应答的重要因子，发展高灵敏、高通量糖蛋白的纳米探针技术和传感分析方法是极具挑战性的生命科学和分析化学前沿课题，纳米簇材料的电致化学发光提供了一个崭新的手段，本项目拟纳米簇材料、电致化学发光技术、分子探针技术有机结合，建立钙粘蛋白的纳米簇ECL探针和传感体系；发展ECL纳米簇的合成新策略，通过水热合成控制、微波辅助和配基交换等手段，制备系列高质量ECL纳米簇；系统研究纳米簇ECL的主要形式、强度与簇直径、表面配基间的关系；以纳米簇的功能化为基础，利用共价和非共价修饰方法，控制合成系列钙粘蛋白ECL纳米簇探针，利用电化学分析手段，较系统地研究纳米簇的生物分子固定及活度保持规律；利用自组装、电化学沉积、层层组装等，建立基于纳米簇的电致化学发光生物传感界面，发展相应的分析方法，为糖蛋白的生物环境下分析提供重要的方法学参考。

Cadherin is an important factor for the intercellular signal transduction and response, It still remain a challenge to develop new sensing method and nanoprobe technique with highly sensitivity and high throughput to study the glucoprotein of cell in life science and analytical chemistry. Fortunately, the nanoclusters as a new kind of electrogenerated chemiluminescence(ECL) reagent provide a new pathway for this. The main propose of this projiect is to establish the nanoclusters ECL probe and sensing systems to combine nano-materials, ECL technique and molecular probe techniques. To develop new strategies for the synthesis of ECL nanoclusters, series of high-quality ECL-nanocluster are prepared by control hydrothermal synthesis, microwave-assisted and ligand-exchange. The relatonship between the ligand of nanoclusters and the ECL models together with their intensity are systematically investigated. Research is carried out based on the function of the nanoclusters, the surface of nanoclusters is introduced into chemical functional groups, then nano-probe for ECL is synthesized using the covalent or non-covalent modify methods, and systematic studied the biomolecule immobilizaton and activity on surface of nanoclusters. With the aid of the technology such as self assembly, layer-by-layer and electrochemiacl deposition, the new type of ECL sensing interface based on nanoclusters can be build, which can not only be used for highly sensitive and in situ analysis of complex biological samples, but also provide an important way for the detection of glucoprotein of cell.