聚合物基底表面杨氏模量的精确调控与表征是力生物学研究领域面临的主要技术挑战，是认识细胞−力学微环境相互作用机制的关键方法基础。本项目基于聚对二甲苯微机电系统（Parylene MEMS）工艺，拟解决跨尺度Parylene C“扩散−淀积”机制、Parylene C填充聚合物表面等效杨氏模量的荧光定量读取映射关系等关键科学问题；建立聚合物表面超薄（等效厚度<10 nm）Parylene C填充工艺、聚合物表面力学性能精确调控以及Parylene C自发荧光可控增强等关键技术；实现杨氏模量1−1000 kPa范围可调、连续梯度及离散阵列变化、空间分布精度优于5 μm的聚合物表面力学性能调控以及杨氏模量可视化原位表征方法。有望在单/亚细胞水平实现细胞−力学微环境相互作用的精细调控，为力生物学基础研究及相关疾病机制研究等提供有力工具。

Precise modulation and characterization of Young’s modulus on the polymer surface is a significant technical challenge in the mechanobiology study, and the key method to understand the interaction mechanism between cells and mechanical microenvironment. Based on the Parylene MEMS (microelectromechanical system) process, this project will establish the ultra-thin (nominal thickness<10 nm) Parylene C-caulked polymer process, techniques for Young’s modulus modulation on polymer surface and controllable enhancement of Parylene C autofluorescence intensity. Two key scientific problems, the multi-scale diffusion-deposition model of Parylene C and the numerical correlation between the Parylene C autofluorescence intensity and Young’s modulus value, will be solved. Then, the precisely patternable modulation of mechanical property and controllable enhancement of Parylene C autofluorescence intensity will be studied. Finally, a method for mechanical property modulation with a large range (1−1000 kPa), complicated patterns (continuous gradient and discrete patterns) and a high precision (spatial resolution better than 5 μm), and visualized characterization of Young’s modulus will be developed. It is promising to realize a precise modulation of interaction between cells and mechanical microenvironment at the single/sub cellular level. Meanwhile, the achievement from this project will also provide a powerful tool for the study of basic mechanobiological science and physiological mechanism of related major diseases.