鱼油是海水鱼虾不可或缺的饲料原料，然而鱼油极易氧化，氧化鱼油对动物肠道的损伤极易发生且危害巨大。课题组分离出的乳酸乳球菌CAMT22361的体外抗氧化效果已得到验证，但其对动物氧化应激肠道的保护机制尚不明确。本项目拟通过氧化鱼油诱导珍珠龙胆石斑鱼肠道应激，通过比较正常组、肠道氧化应激组及CAMT22361干预浓度、干预方式及作用时间处理组的石斑鱼生长、免疫、肠道粘膜抗氧化指标和粘膜炎症因子指标、肠道形态及菌群谱的影响，阐明乳酸乳球菌与肠道菌群和肠道氧化的关系。利用宏基因组、转录组和代谢组手段研究正常组、氧化应激组和乳酸菌干预组肠道菌群结构与功能基因、菌群和黏膜代谢物、黏膜表达基因、菌群和机体代谢通路的差异，筛选出差异代谢物和差异表达基因。通过宏基因组代谢通路、基因功能与菌群代谢产物关联分析及黏膜转录组代谢通路、表达基因与黏膜代谢物关联分析，共同揭示CAMT22361的肠道抗氧化应激机制。

Fish oil, which rich in polyunsaturated fatty acids, is an indispensable feed material for marine fish and shrimp. However, fish oil is easily oxidized, and oxidized fish oil can easily damage the intestinal tract of aquatic animals. As a probiotic, lactobacillus has attracted much attention for its antioxidant function. Therefore, it is of great significance to reveal the protective mechanism of Lactococcus lactis CAMT22361 on intestinal tract under oxidative stress in order to reduce the harm of oxidized oil to aquatic animals. In this study, oxidized fish oil was used to induce intestinal stress in grouper. To clarify the relationship between Lactococcus lactis CAMT22361 with intestinal flora and intestinal oxidation by compare the growth, immunity, antioxidant index of mucosa, inflammatory factor index of intestinal mucosa, intestinal morphology and microflora spectrum of normal group, intestinal oxidative stress group and Lactococcus lactis CAMT22361 groups that treated with concentration, mode and time. Metagenomic, transcriptome and metabolome methods are used to study the differences in intestinal flora structure and functional genes, bacterial and mucosal metabolites, mucosal expression genes, bacterial flora and metabolic pathways in normal, oxidative stress and lactic acid bacteria intervention groups , and the differential metabolites and expressed genes are screened. Through the macrogenome metabolic pathway, gene function and microbial association analysis, and mucosal transcriptome metabolic pathway, expression gene and mucosal metabolite association analysis, the mechanism of anti-oxidative stress of CAMT22361 intervention in intestinal tract is revealed.