调控NLRP3炎小体的异常活化已成为干预炎症性肠病(IBD)及其相关肠癌变的一个潜在治疗靶点。过量的ROS是诱导NLRP3炎小体活化的要因之一，而降低肠组织内ROS水平可明显缓解小鼠实验性IBD及其相关的结肠癌变。芸薹属紫色菜蔬(紫菜薹、紫芜菁、紫甘蓝和羽衣甘蓝)是一类富含强抗氧化和抗炎能力物质花青素的十字花科特色蔬菜，其对IBD的干预效果还未被系统研究过。前期研究中，我们发现芸薹属紫甘蓝能明显抑制人白血病THP-1细胞中NLRP3炎小体活化后主要生理效应分子IL-1β和IL-18的分泌，且明显抑制结肠炎小鼠血清及结肠组织中IL-1β的表达。因此，本研究设想以体外化学法研究样品的抗氧化和抗炎效果，结合细胞模型，并利用三硝基苯磺酸和葡聚糖硫酸钠分别诱导的小鼠实验性IBD模型，揭示这四类芸薹属紫菜蔬对IBD小鼠中NLRP3炎小体的分子调控机制，为其在人类IBD的防治层面提供一定的科学依据。

Recently, modulation of the NLRP3 inflammasome overacrivation is a potential therapeutic target in preventing inflammatory bowel disease(IBD) and it associated colonic carcinogenesis. Some studies suggested the overgenerated ROS plays a very important role during the NLRP3 inflammasome activation. Decreasing the colonic ROS levels was able to reduce the experimental mice IBD and IBD-associated colon carcinogenesis. Purple Brassica rapa L vegetables, including Brassica campestris L. var. purpurea Bailey, purple Brassica rapa var. rapa, Brassica oleracea var. capitata f. rubra and Brassica oleracea L. var. acephala DC were enriched in anthocyanisns which with a highly antioxidant and anti-inflammatory activity. However, the IBD preventive effect of these purple vegetables has not been study. In our previous studies, we found the Brassica oleracea var. capitata f. rubra (red cabbage) was significantly reduced the secretion levels of IL-1β and IL-18, which are both the major physiological effector during the activation of NLRP3 inflammasome in human monocytic leukemia THP-1 cells. In addition, red cabbage also significantly decreased the serum levels of IL-1β, and reduced its colonic mRNA expressions on the DSS-induced colitis mice. Based on these results, this study was first to investigate the antioxidant and anti-inflammatory activities of these purple vegetables using in vitro test and in cell models. Secondly, we focused on investigating the effects of purple purple Brassica rapa L vegetables on regulating the NLRP3 inflammasome activation on 2,4,6-trinitro picrylsulfonic acid(TNBS) and dextran sulfate sodium(DSS) induced inflammatory bowel disease mice model, respectively. The aim of this study was to reveal the molecular mechanisms of these purple vegetables on reregulating the NLRP3 inflammasome activation on experimental IBD mice model. In addition, these results from the present study will provide some scientific evidences for human IBD prevention and clinical therapy.