因男性生育年龄增加而导致的生精障碍与生育缺陷越来越引起人们的关注,但目前仍缺乏有效防治措施。补肾方药具有良好的延缓衰老与改善男性生殖功能的作用，但其延缓睾丸生殖衰老的作用与机制仍有待研究。因年龄增长导致睾丸精原干细胞niche变化是生殖功能衰退的主要原因，而let-7-Imp轴的变化是其主要分子机制。相关研究与我们前期研究结果均显示，补肾方药具有调节干细胞niche与干细胞增殖分化的作用；但补肾方药能否通过调节精原干细胞niche进而延缓睾丸生殖衰退仍有待研究。本项目拟先采用流式细胞分析、PCR array、干细胞移植技术，研究补肾方药（五子衍宗丸、淫羊藿总黄酮）对衰老大鼠精原干细胞niche与干细胞功能的调节作用，再采用SiRNA、Realtime PCR技术，研究补肾方药对睾丸内let-7-Imp轴变化的影响，进而探讨补肾方药延缓睾丸生殖衰退的作用机制，为临床防治生殖衰老提供新的思路。

More and more people concern the reproductive disorders and birth defects caused by aging of the male reproductive system, but there is no effective treatment. Tonifying kidney recipe could play a significant role in delaying aging and improving male reproductive function, but its mechanism on protecting testis reproductive function are still elusive. Some research showed that aging caused the change of testicular stem cell niche was the main mechanism of affecting the spermatogonial stem cell function, while the aging associated the let-7-Imp axis is the main molecular mechanism. The related research and our preliminary results showed that some recipe could regulate stem cell niche and stem cell function; but whether it could delay reproductive senescence by regulating spermatogonial stem cells niche remain to be studied. This project intends to research the effects of Tonifying kidney recipe ( Wuzi Yanzong decoction, total Flavones of Epimedium ) on the aging rat spermatogonial stem cell niche and its function using flow cytometry, PCR array, stem cell transplant technology, then the SiRNA, Real time PCR technology are utilized to research the changing of the let-7-Imp axis in the testis. Through investigating the effect and mechanism of Tonifying kidney recipe on the delaying reproductive senescence, It will provide new ideas for clinical intervention of reproductive aging.