唾液蛋白在灰飞虱取食、传毒中的作用和机理研究

植食性昆虫的唾液包含多种与取食相关的消化、水解酶类，还包含一些与病原微生物传播相关的蛋白和能够调控植物防御反应的因子，虽然唾液在昆虫、寄主植物、病原微生物三者的相互关系中发挥着重要作用，但在分子层面的研究还知之甚少。本项目以传播多种病毒病的重要水稻害虫灰飞虱为研究对象，利用取食人工饲料和水稻的带毒与不带毒灰飞虱唾液腺平行转录组测序和唾液蛋白组技术，筛选可能与灰飞虱取食和传毒相关的唾液蛋白，然后利用Western blot、RNAi、外源蛋白渗透法等分子生物学技术确定相关唾液蛋白在取食和传毒中的作用，并从唾液调控植物防御方面解析其作用机理，阐明灰飞虱唾液蛋白的适应性进化机制。

Insect herbivore saliva contains digestive enzymes, as well as other components, such as elicitors or effectors that modify plant defense and proteins related to pathogen transmission. Herbivore saliva plays an important role in the plant–herbivore-pathogen interactions. However, thus far little is known at the molecular level. Therefore, in this study, combined the high-viruliferous L. striatellus saliva proteome, we compared the salivary-gland transcriptomes of three strains of L. striatellus: Virus-free population feeding on artificial diet, high-viruliferous and virus-free populations feeding on rice, to screen out the genes coding salivary proteins responded to feeding and virus transmission. Then these key genes function will be studied by Western blot, RNAi and recombinant active protein infiltration and explained in the way that saliva can modify plant defense. The results will shed light on the molecular mechanisms of the adaptation of L. striatellus.