自体脂肪是软组织缺损的理想填充物，但移植后脂肪再生的不稳定影响了其结果的可控性。脂肪移植后血管网的重建与成熟是维持稳定性脂肪再生的基础。我们在前期研究中发现，在新型脂肪再生模型中，脂肪来源干细胞分化的周细胞与内皮细胞通过信号交互形成了器官特异性血管网（Organotypic Vasculature，OV），保障了稳定的脂肪再生。但脂肪来源干细胞参与血管再生的机制并不明确。进一步的研究发现，OV中的周细胞膜高表达Notch2蛋白，提示Notch2信号与脂肪再生中的血管再生相关联。本研究基于以上发现，拟通过上调和下调Notch2蛋白，观察其下游信号对OV形成的影响，揭示Notch2信号在脂肪再生过程中的作用及调控机制，为脂肪来源干细胞疗法及脂肪再生诱导提供理论基础和实验依据。

Although fat tissue is the most ideal filling material for soft tissue defects, the instability of fat regeneration after transplantation leads to different outcomes.The reconstruction and maturation of vascular network is essential for maintaining stable fat regeneration.We previously found that the adipose-derived stromal cells performed pericytic phenotype form Organotypic Vasculature (OV) through crosstalk with endothelial cells, guaranteeing stable fat regeneration.However, the detail mechanism of how adipose-derived stromal cells involved in vasculogenesis is not clear.Further investigation revealed that the pericyte membrane in OV highly expressed Notch2 protein, suggesting that the Notch2 signal is associated with OV formation in fat regeneration.By investigating the influence of up-regulation and down-regulation of Notch2 protein on the formation of OV, this research is believed to be helpful to reveal the mechanism of Notch2 signal in fat regeneration, which will provide solid experimental evidence for adipose-derived stromal cell therapy and fat regeneration induction.