马铃薯细胞质甘油醛-3-磷酸脱氢酶调控块茎顶端优势机理研究

马铃薯是贵州第三大粮食作物，其种植面积和产量居全国前三，而单产始终低于全国平均水平，播种时块茎芽过度生长是造成这一现象的重要原因之一。调控块茎顶端优势可以调节芽的生长，并影响植株主茎数与产量。研究证实，激素和糖是顶端优势调控信号；顶芽分生组织程序性细胞死亡（PCD）与顶端优势相关，并受到细胞自噬调控。前期研究发现，马铃薯细胞质甘油醛-3-磷酸脱氢酶（StGAPCs）与细胞自噬相关蛋白（StATG3）存在互作，前者基因沉默会导致块茎顶端优势表型与糖含量变化，伴随发生顶芽分生组织细胞自噬。项目通过多重组学研究StGAPCs沉默导致块茎顶端优势表型变化涉及的代谢途径、关键酶和代谢产物，并分析其与细胞自噬、PCD、糖和激素代谢的关系，阐明StGAPCs-StATG3互作介导的细胞自噬途径与PCD及顶端优势的关系，为深入理解顶端优势发生与调控机制提供理论基础，为块茎休眠及萌芽的调控提供基因资源。

Potato is the third largest food crop in Guizhou Provence, and its planting area and production quantity rank among the top three in China. Nevertheless, its yield is always lower than the national average level. Over-development of tuber buds before seeding is one of the important reasons for this phenomenon. Regulation of tuber apical dominance can regulate bud growth and affect the number of main stems and yield of plants. It was confirmed that hormones and sugar are act as signals in apical dominance regulation; apical bud meristem programmed cell death (PCD) is associated with apical dominance and regulated by autophagy. Previous studies have found that there is interaction between cytoplasmic glyceraldehyde-3-phosphate dehydrogenase (StGAPCs) and autophagy-related protein (StATG3) in potato. The silencing of StGAPCs gene lead to changes in the apical dominance phenotype and sugar content of tuber, accompanied by autophagy of apical bud meristem. The metabolic pathways, key enzymes and metabolites involved in the phenotypic changes of tuber apical dominance caused by StGAPCs silence will be studied in this project through Integrated analysis of proteome and metabolome, and their relationship with autophagy, PCD, sugar and hormone will be analyzed also, clarifying the relationship between autophagy of apical bud meristem mediated by StGAPCs-StATG3 and apical bud meristem PCD. The results can be conducive to provide theoretical basis for further understanding the mechanism of apical dominance occurrence and regulation, and genetic resources for the regulation of tuber dormancy and germination.