生物之间的互作关系一直是生态学研究的热点问题。玫红铆钉菇和粘盖乳牛肝菌是一对共同发生的外生菌根（ECM）真菌，主要与松科植物共生，其中玫红铆钉菇子实体仅在粘盖乳牛肝菌出现时才会发生。本项目以玫红铆钉菇、粘盖乳牛肝菌和马尾松为研究对象，采用对峙培养法并结合SEM技术研究纯培养条件下玫红铆钉菇与粘盖乳牛肝菌的互作关系；通过ECM合成并结合形态学和转录组分析研究粘盖乳牛肝菌对玫红铆钉菇与马尾松的共生关系建立的影响；通过观测两种ECM真菌的子实体发育过程以及粘盖乳牛肝菌子实体清除对玫红铆钉菇子实体发生的影响分析粘盖乳牛肝菌子实体发生与玫红铆钉菇子实体发生的关系。综合以上实验结果，从孢子萌发和菌丝体生长、菌根合成和子实体发生角度阐明玫红铆钉菇对粘盖乳牛肝菌的依赖性的机理，明确玫红铆钉菇、粘盖乳牛肝菌与马尾松的互作关系，为进一步揭示外生菌根真菌多样性维持机制和外生菌根共生机理提供理论依据。

Interaction among organisms has always been a hot topic in ecological research. The co-occurrence of Gomphidius roseus and Suillus bovinus, two ectomycorrhizal fungi, is quite common around the world. These two fungi usually form ectomycorrhiza with Pine trees, and the sporocarps of G. roseus could only be found when the sporocarps of S. bovinus are present. In this project, a series of experiments will be conducted to elucidate the interactions among G. roseus, S. bovinus and P. massoniana. The possible effects of S. bovinus on G. roseus in vitro will be verified through the confrontation culture method and combining with SEM analysis. The effects of S. bovinus on ECM synthesis between G. roseus and P. massoniana will be evaluated through ECM synthesis tests combining with morphological and transcriptome analysis. Through the observation of the sporocarp development process of the two fungi and the effects of sporocarp elimination of S. bovinus on G. roseus fruiting, the effects of S. bovinus fruiting on sporocarp genesis of G. roseus will be evaluated. Combining the researches above, the effects of S. bovinus on the spore germination and mycelium growth, mycorrhizal synthesis and sporocarp genesis of G. roseus will be evaluated to elucidate the mechanism behind the observed dependence of G. roseus on S. bovinus. The promising results will be helpful to clarify the interactions among G. roseus, S. bovinus and P. massoniana, and further contribute to elucidate the mechanisms of ECM symbiosis and the sustainable of the diversity of ECM fungi in the wild.