基于外磁驱动的封闭式全自动传染病分子标志物信息采集与分析系统构建与应用研究

COVID-19新型冠状病毒，来势凶猛传染性极强，短时间即迅速漫延，使全球处于恐慌之中。对这类高传染性和高致病性病毒，快速获取其分子特征信息并采取高效防控措施至关重要。由于核酸检测高特异和高灵敏，基于核酸标志物信息获取的传染病病原体检测技术已被广泛应用。但仍需复杂的核酸提取和循环扩增检测过程，且依赖于实验环境和检测人员操作熟练程度，耗时、检测效率低、存在交叉污染和假阳性风险的问题并未根本解决。因此，研究集样本处理、检测和结果报告于一体的封闭式全自动核酸标志物信息快速检测系统，对传染病防控十分重要。本课题拟在已有工作基础上，针对现有封闭式卡盒一体化核酸检测仪技术瓶颈，构建一种全新的外磁场驱动卡盒，通过外部磁力无接触驱动封闭式卡盒内的磁性纳米颗粒，结合先进的扩增与检测技术，准确、快速、便捷地在1小时内完成样本处理及核酸分子标志物信息检测全过程，为完善保障人民生命健康的生物安全体系提供有力支撑。

COVID-19 coronavirus, highly infectious, in a short time spread around the world, resulting in the world in a panic. For this kind of highly infectious and highly pathogenic virus, it is very important to quickly obtain its molecular characteristic information and take effective prevention and control measures. Due to the high specificity and sensitivity of nucleic acid detection, the detection technology of infectious disease pathogen based on nucleic acid molecular marker information has been widely used. However, although it is simpler and faster than the traditional technologies, it still needs to complete the complex process of nucleic acid extraction and cyclic amplification detection. Besides, it also depends on the laboratory environment and the proficiency of the technicians, which is time-consuming and labor-intensive, and the efficiency is relatively low, which also increases the cross-contamination and false positive or negative rate owing to the incompletely closed feature. Therefore, it is very important for the prevention and control of infectious diseases to fabricate a closed, automatic and rapid detection system integrating sample processing, detection and result reporting to obtain the nucleic acid molecular marker information. Based on the techniques usually applied, and aimed at overcoming the bottleneck of the existing integration nucleic acid detection technology based on a fully-enclosed cassette, we will fabricate a novel and fully automatic infectious diseases detection system, based on a fully-enclosed cassette, external magnetically driving, and the recombinase polymerase amplification (RPA) technology to finish the whole procedure including sample processing, qPCR and final result report in 1 hour with high accuracy, rapidness and conveniency. This novel nucleic acid molecular markers information detection strategy will significantly play a very important role in keeping the people safe.