多种中枢神经系统炎症性疾病中都伴随有免疫细胞招募。前期工作中我们建立了中枢神经系统炎症动物模型，首次证实补体关键成分C3和C5是炎症状态下免疫细胞被招募进入中枢神经系统过程中不可或缺的成分，且补体成分并不是通过其趋化属性来介导中性粒细胞招募。我们将进一步使用已建立的活体显微镜技术对C3和C5在免疫细胞进入中枢的招募级联过程中所扮演的角色进行探索。本研究将回答以下问题：1.C3和C5在免疫细胞招募的哪一个过程中（胶质细胞激活，内皮细胞激活）起到了关键作用； 2.参与激活胶质细胞或者内皮细胞的补体成分究竟来自于外周循环还是中枢神经系统，具体分泌来源是哪一类细胞； 3.选择性表达或调控补体激活对免疫细胞招募是否有促进作用,对中枢神经系统炎症是否有影响。我们的工作最终将为各种中枢神经系统炎症性疾病的干预提供有利的方案和理论线索。

Infiltration of immune cells occurs in many types of central nervous system (CNS) inflammatory diseases. Our preliminary data showed that C3 and C5 were critical molecules during neutrophil recruitment in CNS inflammation, and the recruitment was not mediated by its chemotactic properties. Using intravital microscopy, a cutting edge technique, we will explore the roles of C3 and C5 in mediating immune cell recruitment into the CNS and answer the following questions: 1.Are C3 and C5 essential for microglial or endothelial activation in the recruitment cascade? 2. Do these complement molecules activated microglia or endothelium come from peripheral circulation or CNS, what are the exact cells source for them? 3.We will also generate transgenic animals selective expressing these complement components and look at leukocyte recruitment and CNS inflammation. Our research will provide evidence and a useful strategy for interfering those CNS inflammatory diseases with leukocyte infiltration via targeting complement activation.