骨骼肌卫星细胞是一种肌源性干细胞，在骨骼肌的生长、发育及肌肉损伤修复中起着至关重要的作用。先前的研究结果表明：肌卫星细胞的激活受到表观遗传学调控，但DNA甲基化在其中的作用机制尚不明确。为了阐明DNA甲基化对猪骨骼肌卫星细胞增殖分化的影响，本项目拟以五指山小型猪为研究对象，体外分离、培养骨骼肌卫星细胞，分析DNA甲基化在猪骨骼肌卫星细胞增殖分化过程中对成肌因子表达的调控，鉴定DNA甲基转移酶在肌卫星细胞增殖分化中的作用，探讨通过改变成肌因子基因组甲基化模式是否可以开启骨骼肌卫星细胞的增殖分化过程。本项目的实施将为五指山猪生长发育规律、系统选育及矮小机制等方面的研究提供表观遗传学依据，具有重大的科学意义和实践价值。

Satellite cell is a kind of myogenic stem cells,which plays an important role in muscle development and injury repair.Previous reports have demonstrated that epigenetic control of skeletal muscle satellite cell activation.However,little is known about DNA methylation mechanism of muscle satellite cells.To clarify the role of DNA methylation in proliferation and differentiation of porcine skeletal muscle satellite cells, we chose Wuzhishan miniature pigs as the subject in this study, dissociated and cultured porcine skeletal muscle satellite cells in vitro, investigated the effects of DNA methylation on the porcine skeletal muscle satellite cell proliferation and differentiation, detected the expression pattern of DNA methyltransferase and analysed the effect of DNA methylation on the regulatory factors during skeletal muscle satellite cell proliferation and differentiation. This study could be provided some useful Epigenetic basis principles for growth and development, system selection, short mechanism of Wuzhishan miniature pigs.