水稻纹枯病是由立枯丝核菌引起的一种真菌性病害，是水稻生产中最严重的病害之一。Burkholderia sp. JP2-270是本研究室自主分离得到的一株能有效拮抗水稻纹枯病菌的生防细菌。前期研究发现LysR类型的转录调控蛋白BysR对JP2-270抑制水稻纹枯病菌起关键作用，本申请项目拟通过转录组测序分析、分子生物学技术和代谢分析的方法研究BysR在JP2-270抑制水稻纹枯病菌方面所扮演的角色。本申请项目预研结果显示，一种糖肽类物质-occidiofungin的合成基因簇受BysR调控。项目需解决的问题包括：1. 证实JP2-270能够合成occidiofungin类物质，且该物质对水稻纹枯病具有抑制作用。2. 明确JP2-270中的LysR类型的全局转录调控蛋白-BysR调控occidiofungin的合成模式。

Rice sheath blight is a fungal disease caused by Rhizoctonia solani and one of the most serious diseases in rice production. Burkholderia sp. JP2-270, isolated from the rhizosphere of rice, is a biocontrol bacterium which can effectively inhibit the growth of R. solani. Previous studies have found that BysR, a transcriptional regulatory protein of LysR type, plays a key role in the inhibition of Burkholderia sp. JP2-270 against Rhizoctonia solani. We plan to study the role of BysR involved in the antagonism of JP2-270 against R. solon through transcriptome, molecular biology and metabolic analysis in this study. Pre-research of this study revealed that the synthetic gene cluster of occidiofungin, a glycopeptide, is regulated by BysR. Occidiofungin has antifungal activity, and its synthesis is regulated by LuxR-type pathway-specific transcription regulators AmbR1 and AmbR2. At present, no studies have shown that occidiofungin synthesis is also regulated by other types of transcriptional regulators. Therefore, the problems that need to be solved in this study are as follows. First, we need to confirm that JP2-270 can synthesize occidiofungin-like substances and that they can inhibit rice sheath blight. Second, we need to clarify the synthesis mode of occidiofungin regulated by BysR. This study will expand the research scope of occidiofungins on fungal diseases, and enrich the regulatory network of the this compound. This study provides a theoretical basis for optimizing the synthesis of occidiofungins-like substances.