RNA介导的高抗和多抗植物病毒病育种新策略的研究

批准号:
39970485
项目类别:
面上项目
资助金额:
13.0 万元
负责人:
温孚江
依托单位:
学科分类:
C1401.植物病理学
结题年份:
2002
批准年份:
1999
项目状态:
已结题
项目参与者:
傅连海、郭兴启、张松、宋云枝、朱常香
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中文摘要
以PVY、CMV及烟草为材料,研究RNA介导抗病性的特点及遗传稳定性,研究不同有效cDNA片段组合连接后转化植物对抗病性的影响,探索以此来增强转基因植物对病毒的抗性及抗病性持久性、扩展抗病范围(同时抗几个流行病毒株系)以及多抗(同时抗两种以上病毒)基因工程策略的可行性及应用价值。
英文摘要
cDNAs covering the full genome of potato virus Y (PVY) were cloned by transcription-ploymerase chain reaction. Point mutation techniques were used to get rid of any possible start codons in the cDNA fragments. Plant expressing vectors containing segments from different region, different length and different slicing strategies were constructed. The plant expressing vectors were transformed into tobacco. Molecular hybridization analysis and resistance identification showed that the transformed cDNA fragments were expressed successfully at the transcription level, but no translated productions were examined. The transgenic plants showed high resistance to PVY infection. These results revealed that the resistance to PVY infection was mediated at RNA level. The resistance mediated by the cDNA from 3' region of the PVY CP gene was much stronger than that of 5' region, and the minimum length of cDNA was 200bp for resistance initiation. The results also showed that the best construction is the inverted repeat, from which the transcript could form hairpin construction. Based on these results, plant expressing vectors containing the cDNAs from the 3' regions of both PVY and potato virus X (PVX) CP genes were constructed in such way in which inverted repeats of their transcripts could be formed. The transgenic plants transformed with such constructs showed high resistance to both PVY and PVX infections.
专著列表
科研奖励列表
会议论文列表
专利列表
hpRNA的茎环长度和比例对RNA介导病毒抗性的影响
- 批准号:30471139
- 项目类别:面上项目
- 资助金额:22.0万元
- 批准年份:2004
- 负责人:温孚江
- 依托单位:
RNA介导的病毒抗性的产生、信号传导及应用研究
- 批准号:30270875
- 项目类别:面上项目
- 资助金额:22.0万元
- 批准年份:2002
- 负责人:温孚江
- 依托单位:
外源基因在转基因植物内遗传规律的研究
- 批准号:39470402
- 项目类别:面上项目
- 资助金额:8.0万元
- 批准年份:1994
- 负责人:温孚江
- 依托单位:
国内基金
海外基金
