糖尿病视网膜病变（DR）的机制和治疗是目前研究的热点和难点。我们的前期研究发现巨噬细胞对DR的微血管异常和神经细胞损害都有相关作用。本研究拟采用模拟DR环境的多种动物模型和细胞模型，应用视网膜铺片、免疫组化、RT-PCR、Western blot、TUNEL凋亡检测法、经上丘逆行标记视网膜神经节细胞和细胞共培养等方法，研究巨噬细胞在DR中导致视网膜微血管异常和神经细胞损害的相关机制；在此基础上，对各种不同的DR动物模型及细胞模型进行PEDF干预，观察其对巨噬细胞调控后视网膜的微血管改变及神经细胞凋亡变化等情况，探讨PEDF是否可以作为抑炎因子来调节DR时巨噬细胞的功能。本课题旨在获得关于PEDF可以作为一种抑炎因子，在DR时通过调控巨噬细胞的功能来改善视网膜微血管病变和神经细胞损害的证据，从而丰富DR治疗的靶点及理论依据。

The mechanisms and treatment of Diabetic retinopathy (DR) have received increasing concerns and are difficult problems to resolve in current studies. Our previous studies found that macrophages are involved in microvascular abnormalities and neurological damages in DR. Therefore, animal and cell models mimicking DR pathophysiologic environment will be applied to investigate the roles of macrophages in microvascular and neurological abnormalities in DR, by means of retinal flatmounts, immunohistochemistry, RT-PCR，western blot assays, TUNEL staining technique，superior colliculus retrograde labeled retinal ganglion cells and co-cluture of different cells. Furthermore, pigment epithelium derived factor (PEDF) will be used as an intervention to detect its regulatory effect of macrophages on microvascular abnormalities and neurological damages. As a result, this study aims at whether PEDF can be used as an anti-inflammatory factor to modulate functions of macrophages in DR to ameliorate the microvascular and neurological abnormalities.The expected results of the project will enrich the therapeutical target and theoretical basis of DR.