近年来的研究已将关于狄斯瓦螨和蜜蜂残翅病毒（DWV）协同作用关系的认识推向了一个新的高度。其中重要进展包括DWV分型的确立以及狄斯瓦螨促进了DWV在西方蜜蜂体内的增殖和优势毒株的确立。本项目将结合国际最新研究进展和借助国内东、西方蜜蜂共存的优势条件，比较研究狄斯瓦螨和DWV在感染东方蜜蜂和西方蜜蜂时的作用模式。将主要通过调查分析和人工转染实验，阐明：（1）我国中华蜜蜂（中蜂）和意大利蜜蜂（意蜂）蜂群携带的DWV的多样性及分布模式；（2）DWV不同优势变体对中蜂和意蜂工蜂蛹发育和成年工蜂健康的影响；（3）人工转染条件下狄斯瓦螨和DWV在中蜂和意蜂工蜂蛹和雄蜂蛹上作用关系的异同，及不同种、不同性别蜜蜂的免疫应答异同。本项目将建立对狄斯瓦螨、DWV和蜜蜂的三维互作关系更系统全面的认识，也将有助于揭示蜜蜂抗狄斯瓦螨和抗DWV病毒的机制，对养蜂生产上狄斯瓦螨和DWV的防治也有重要指导意义。

Recent advances has dramatically deepened our knowledge on the synergistic effect of Varroa destructor and Deformed wing virus on honeybee health. These important advances include the classification of DWV variants and the finding that V. destructor infestation has a significant effect on viral load and strain diversity of DWV on honeybees. Based on the recent advances, taking advantage of the co-occurrence of Apis mellifera and Apis cerana in China, we compare the interaction of V. destructor and DWV on different honeybee hosts. With investigation on field colonies and artificial infection experiments, we are aiming to demonstrate (1) diversity of DWV infecting A. ceana cerana and A. mellifera ligustica in China and the distribution of different variants; (2) effect of Chinese DVV master variants on the development of worker pupae and the health of adult bees of A. ceana cerana and A. mellifera ligustica; (3) similarities and differences of the interaction of V. destructor and DWV when infecting A. ceana cerana and A. mellifera ligustica worker and drone pupae in artificial infection experiments and the corresponding immune response of honeybees. This project will deepen our understanding on the interaction among V. destructor, DWV and honeybees and is helpful to unveil the mechanisms to resist Varroa parasite and DWV of honeybees. The research is also of significance toward fighting against V. destructor and DWV in beekeeping practice.