在细胞分化过程中，基因表达呈选择性和高度协同性。精确的基因协同表达调控是细胞分化的分子基础，开展细胞分化过程中基因协同表达调控分子机制的研究具有重要的基础理论意义。我们认为染色质远程相互作用参与调控基因的协同转录。为了研究基因协同转录调控的分子机制，我们拟利用染色体构象俘获技术、并结合高通量的3C/4C-seq测序技术，研究早幼粒细胞诱导成熟过程中染色质动态上的相互作用和空间组织；同时分析研究染色质相互作用与基因座位上所在基因协同转录水平之间的关联性，认识更多潜在远距离顺式调控元件与靶基因之间的染色质相互作用事件；并利用顺式调控元件俘获技术和报告基因调控表达系统，研究与靶基因发生相互作用位点作为顺式调控元件的生物学功能，进一步研究这些基因协同转录调控的分子机制。本研究将促进对细胞发育与分化分子机制的认识。

During the course of cell differentiation, the genes expressions show selectivity and high cooperativity. The genes precisely cooperative expression and regulation are the molecular basis for cell differentiation. It is obviously great significance in the basic science theories to investigate the molecular mechanisms of the genes cooperative expression and regulation during the cell differentiation. We speculate that the long-range chromatin interaction participate in regulating the genes cooperative transcription. In order to investigate the molecular mechanisms of the genes cooperative transcription and regulation, we plan using the techniques of chromosome conformation capture (3C), combining with the high-throughput 3C/4C-seq sequencing techniques to investigate the chromatin dynamic interaction and spatial organization of the promyelocytic cells during induced-maturation; and we also analyze the genes on the interacted locus transcription level might be relevant with the chromatin interactions, to learn the much more cases of the chromatin interactions between the potential long-range cis regulate elements and the target gene loci. We farther analyze the biological function of the potential long-range cis regulate elements which interacted with the target gene loci using the techniques FAIRE (Formaldehyde-Assisted Isolation of Regulatory Elements) and reporter gene expression and regulation system, and farther to investigate the potential molecular mechanisms of the genes cooperative transcription and regulation. This project will help us to understand the molecular mechanisms of the cell development and differentiation.