萝藦科植物有性生殖过程特殊，是研究繁殖生态学的经典植物。通关藤（Marsdenia tenacissima）属萝藦科植物，是一种重要的抗癌药物的原料药材。在自然种群中，通关藤有花多果少的典型生殖现象，目前尚未有人关注其有性生殖过程及低坐果率方面的研究。本项目以萝藦科牛奶菜属通关藤为研究材料。主要研究以下内容：（1）开花物候、花部结构、访花昆虫与传粉的关系；（2）比较不同花粉种类、数量对通关藤坐果率的影响；（3）资源限制及遗传多样性对坐果率的影响。本项目研究结合花粉和资源限制实验及遗传多样性，探讨其与坐果率的关系，并对现有各种“假说”进行验证补充；这将丰富萝藦科植物传粉生物学基础理论，也为通关藤常规育种提供科学依据。

The plant of Asclepiadaceae shows a special process of sexual reproduction, which is a classical material for a study on reproduction ecology. Marsdenia tenacissima, belonging to Asclepiadaceae, is a medicinal material for making anticancer drug. In natural condition, M. tenacissima show a reproductive pattern of mass flowering and few fruiting. Nowadays, little work has been made to elucidate sexual reproduction and low fruit set of M. tenacissima. In this project, M. tenacissima would be used to investigate the reproductive pattern of mass flowering and few fruiting. The followings would be examined: (1) flowering phenology, Floral structure, a relationship between pollinators and pollination; (2) the effects of pollen resource and quantity on fruit set; (3) the effect of resource limitation and genetic diversity on fruit set. In the project, a relationship between pollen and resource limitation and low fruit set would be discussed, and many hypotheses would be tested. This project will add new information on pollination biology of the plant of Asclepiadaceae, and will provide a scientific base on conventional breeding of M. tenacissima.