布鲁氏菌病是一种由布鲁氏菌引起的严重威胁公共卫生安全的人畜共患传染病。目前研究表明，铁离子摄取系统是布鲁氏菌重要的毒力因子，在其致病过程中通过一套精准的铁应答机制调控铁的摄取，从而逃避宿主的“营养免疫”。RirA和Irr是布鲁氏菌重要的铁应答调节因子，然而其对铁应答的精确调控分子机理及其在致病过程中的功能作用目前仍不清楚。因此，本研究以RirA和Irr为切入点，利用细菌转录组学、比较蛋白质组学和CHIP-seq技术，筛选验证受RirA和Irr调控的基因与蛋白，并预测参与该过程的关键sRNA和mRNA分子以及其调控的作用靶点，深入研究RirA和Irr调控的铁应答通路；同时通过基因敲除技术研究RirA、Irr及其调控的关键分子在布鲁氏菌黏附入侵、胞内繁殖等致病过程中的功能作用，全面阐述铁调节因子Irr和RirA对布鲁氏菌铁应答的调控机制及其对布鲁氏菌致病性的影响。

Brucellosis is a zoonotic disease caused by the genus Brucella. It is widely popular in the world and affects the development of the healthy and sustainable livestock. Brucella is an intracellular parasitic bacteria, causing inflammation (arthritis, orchitis, endocarditis, etc.) and reproductive system disorders (such as abortion, infertility, etc.).The Study show that iron uptake system is important for Brucella virulence factor by the accurate iron regulation mechanism. Therefore, this study will constructe Brucella RIRA and IRR gene mutant strain and observe its phenotype and Virulence Variation in different concentrations of iron. The genes associated by RirA/Irr will be screened by CHIP-seq, and be verified function in the iron response. The sRNA and mRNA regulated by RirA and Irr were verificated through RNA-Seq. The target genes of regulated sRNA were predicted and identified through bioinformatics, and the regulatory pathway of iron uptake system was intensive studied.