皮质-纹状体-丘脑-皮质（CSTC）回路神经递质失衡是多发性抽动症（TS）的主要病机，而CSTC中直接通路易化作用增强扮演十分重要的角色。前期对单胺及氨基酸类递质的研究发现，其表达异常可间接兴奋直接通路，诱导TS动物模型刻板行为发生。基于该通路中特异性神经肽和酶系为易化作用提供物质基础，本课题提出健脾止动汤抗抽动机制与抑制纹状体cAMP/PKA系统中DARPP-32（Thr-34）过磷酸化有关。综上，拟以IDPN建立TS大鼠模型；采用ELISA、Western blot检测大鼠纹状体cAMP、PKA、DARPP-32和DARPP-32（Thr-34）含量；原位杂交、RT-PCR检测pDYNmRNA、DARPP-32mRNA表达。从行为学、蛋白、核酸水平探讨健脾止动汤对纹状体pDYN过表达及cAMP/PKA系统介导DARPP-32（Thr-34）位点磷酸化的阻抑机制，为临床应用提供实验依据。

One of the main pathogenesis is thought to be an imbalance in neurotransmitters located in CSTC loop in Tourette syndrome (TS). Preliminary studies on monoamines and amino acids have shown that abnormal expressions of these neurotransmitters accompanied with their receptors promote the direct pathway mediately, which further induce the occurrence of stereotyped behavior in TS models. Considering the specific neuropeptides and enzyme systems in the direct pathway provide the material basis for the facilitatory effect, we put forward the hypothesis that Jianpizhidong Decoction involves in regulating the phosphorylation of DARPP-32 mediated by the cAMP/PKA System in the striatum of TS models. In this project, we will build up TS model rats by intraperitoneal injecting IDPN, detect the contents of cAMP, PKA, DARPP-32 and DARPP-32 (Thr-34) in the striatum by means of ELISA and Western blot; test expressions of pDYNmRNA and DARPP-32mRNA applying in situ hybridization and RT-PCR. We are aiming to explore the suppression mechanisms of Jianpizhidong Decoction related to overpression of pDYN and the phosphorylation of DARPP-32 (Thr-34) mediated by the cAMP/PKA System, provide experimental and theoretical supports for further applications in clinic.