lncRNA H19靶向DKK1激活Wnt/β-catenin信号通路促骨髓间充质干细胞成骨分化在电针抗骨质疏松中的机制研究

骨质疏松症发病率高，医疗花费高昂。课题组既往研究显示电针能调控Wnt/β-catenin信号通路抗骨质疏松，但具体调控机制尚不明确。lncRNA与成骨细胞分化密切相关，结合课题组前期研究结果，课题组提出：电针通过上调lncRNAH19靶向抑制DKK1激活Wnt/β-catenin信号通路的机制促进成骨细胞分化，从而起到促进骨形成、抗骨质疏松的作用。在体外，观察上调/下调lncRNAH19对成骨细胞分化、Wnt/β-catenin信号通路的影响；在体内，观察电针对lncRNAH19过表达及低表达去卵巢大鼠骨质疏松动物模型血清骨形成标志物、骨密度、骨组织形态计量学指标、骨生物力学性能及Wnt/β-catenin信号通路的基因与蛋白表达的影响；探讨电针治疗骨质疏松症的作用机制，为电针治疗骨质疏松症提供理论基础，丰富和发展中医“ 肾主骨”理论的科学内涵。

The incidence of osteoporosis is high and the medical cost is large. Evidences show that electroacupuncture inhibit osteoporosis through regulating Wnt/β-catenin signaling pathway. lncRNA has emerged as a promising target for the treatment of osteoporosis in recent years. On basis of the results of our previous studies, we hypothesize that lectroacupuncture could prevent ovariectomy-induced bone loss through regulating lncRNA H19-DKK1-Wnt/β-catenin signaling pathway. The objective of this study is to investigate the possible mechanisms by which electroacupuncture treats osteoporosis. For this purpose we will examine the effects of electroacupuncture on serum biochemical markers, bone mineral density,bone microarchitecture and bone biomechanical properties, and further assess the mRNA and protein expressions of lncRNA H19-DKK1-Wnt/β-catenin signaling pathway in the OVX rats. Elucidation of the precise mechanisms by which electroacupuncture works can help controlling osteoporosis and eliciting the theory of “Kidney control bone” which is an important theory of Traditional Chinese medicine.