进样系统是决定电感耦合等离子体质谱（ICP-MS）单细胞分析性能的关键因素。然而，现有ICP-MS进样系统无法实现单细胞样品的稳定进样，且各部件间的接口较多，死体积大，样品传输效率低，这极大地限制了ICP-MS单细胞分析的可靠性。本研究拟利用3D打印结合计算机辅助设计研制适用于单细胞分析的一体化ICP-MS进样系统：（1）利用Solidworks软件设计单液滴生成、样品雾化和传输模块，并对各模块进行理论模拟和优化，对模块进行一体化设计；（2）利用高精度3D打印系统对其进行加工制造，并对打印参数和打印材料进行优化；（3）对研制的进样系统进行单细胞分析性能测试，并优化使用条件，使其能够实现高效、稳定地ICP-MS单细胞分析。本项目旨在提供一种一体化ICP-MS进样系统用于单细胞分析，并为3D打印技术应用于小型分析器件及相关组件的研制提供研究思路。

Sample introduction system is the key factor which determines the performance of ICP-MS for single-cell analysis. However, current ICP-MS sample introduction system cannot realize stable introduction of single-cell samples. Tedious interfaces between different components make current system owns large dead volume and low sample transmission efficiency, which greatly limits the reliability of ICP-MS single-cell analysis. This proposal intends to utilize the capability of 3D printing for personalized customization and precision manufacturing, combined with computer-aided design to develop an integrated ICP-MS sample introduction system suitable for single-cell analysis: (1) Utilize Solidworks software to design the droplet generation, sample spray and sample transport modules, conduct theoretical simulation and optimization on them and integrate optimized modules; (2) Utilize high-precision 3D printing system to manufacture the system. And optimize the printing parameters and printing material; (3) Test the single-cell analysis performance of the developed system and optimize the working conditions to enable efficient and stable ICP-MS single-cell analysis at low injection flow rates. This study aims to provide a new type of integrated ICP-MS sample introduction system for single cell analysis. Besides, this study is also expected to provide ideas for the application of 3D printing technology to the development of small analytical devices and related components.