冬瓜果皮和果肉颜色关键基因的克隆及调控机理分析

在冬瓜品质性状中，果皮颜色和果肉颜色均为影响冬瓜外观和食用品质的最重要性状，因而相关基因的克隆及调控机理分析对冬瓜品质育种具有重要意义。本研究以深绿色果皮与黄白色果皮、绿色果肉与黄白色果肉的冬瓜亲本为材料，构建2个F2群体，基于全基因组重测序技术对2个F2子代群体所有单株及亲本进行重测序，根据重测序结果开发标记并构建高密度遗传图谱，对控制冬瓜果皮和果肉颜色关键基因进行QTL定位，获得候选基因。利用时空表达、原位杂交、亚细胞定位等技术手段分析候选基因的表达模式，通过过量表达和RNA干扰转基因冬瓜植株的果皮和果肉颜色表型进行候选基因的功能分析。同时结合转录组学、蛋白质组学以及代谢组学分析冬瓜果皮颜色和果肉颜色的分子调控机理，为今后冬瓜在果皮颜色和果肉颜色方面的定向育种提供理论依据。

Among the quality traits of wax gourd, Pericarp color and flesh color are the most important traits that affect the exterior and taste quality. Gene Cloning and analysis of regulation mechanism of Pericarp and Flesh color Is important for the quality breeding in wax gourd. In this study, the parents with dark green pericarp and yellow-white pericarp, green flesh and yellow-white flesh were used as materials. 2 F2 populations would be constructed. Based on the whole genome re-sequencing technology, all plants of 2 F2 progeny populations and parents would be re-sequenced. Markers will be developed and high-density genetic maps will be constructed based on the re-sequencing results. QTL mapping of key genes controlling the pericarp and flesh color of wax gourd should be carried out to obtain candidate genes. Expression patterns of candidate genes will be analyzed by means of spatio-temporal expression, in situ hybridization, subcell positioning, etc. Functional analysis of candidate genes is carried out by overexpression and RNA interference of the color phenotype of the pericarp and flesh of transgenic wax gourd. At the same time, transcriptome, proteomics and metabolomics are combined to analyze the molecular regulation mechanism of pericarp color and flesh color of wax gourd. The results can provide a theoretical basis for orientation breeding of pericarp and flesh color in wax gourd.